Protein Info for PP_4427 in Pseudomonas putida KT2440

Annotation: Amino acid ABC transporter, permease protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 218 transmembrane" amino acids 12 to 35 (24 residues), see Phobius details amino acids 47 to 69 (23 residues), see Phobius details amino acids 81 to 99 (19 residues), see Phobius details amino acids 131 to 149 (19 residues), see Phobius details amino acids 183 to 202 (20 residues), see Phobius details TIGR03004: ectoine/hydroxyectoine ABC transporter, permease protein EhuC" amino acids 2 to 216 (215 residues), 378.7 bits, see alignment E=9.5e-118 TIGR01726: amino ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family" amino acids 5 to 104 (100 residues), 95.9 bits, see alignment E=1.7e-31 PF00528: BPD_transp_1" amino acids 27 to 209 (183 residues), 61.7 bits, see alignment E=4.1e-21

Best Hits

Swiss-Prot: 46% identical to Y4TF_SINFN: Probable amino-acid ABC transporter permease protein y4tF (NGR_a01530) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K02029, polar amino acid transport system permease protein (inferred from 100% identity to ppu:PP_4427)

Predicted SEED Role

"amino acid ABC transporter, permease protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EM7 at UniProt or InterPro

Protein Sequence (218 amino acids)

>PP_4427 Amino acid ABC transporter, permease protein (Pseudomonas putida KT2440)
MADLLPLMLQGAWVTLQITFFGSLLAIVAATLAALGKMSTWRPLRWFCVVYIEIFRGTSL
LVQLFWLFFVLPLPPFNLELSPYTVAIVGLGLHIGAYGAEIMRGAINSVAKGQYEACTAL
NFRPFTRFRRIILPQALLAAMGPGTNLLIELLKNTSLVSLITLADLSFRARQLDQATFET
LKVFALALLMYFVMAQAINLAMRRLERHLARGRVQGSL