Protein Info for PP_4388 in Pseudomonas putida KT2440

Annotation: Flagellar hook protein FlgE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 TIGR03506: flagellar hook-basal body protein" amino acids 1 to 422 (422 residues), 312.5 bits, see alignment E=2.8e-97 PF00460: Flg_bb_rod" amino acids 4 to 33 (30 residues), 30.8 bits, see alignment (E = 4.5e-11) PF22692: LlgE_F_G_D1" amino acids 85 to 148 (64 residues), 44.4 bits, see alignment E=3e-15 PF07559: FlgE_D2" amino acids 163 to 321 (159 residues), 97.2 bits, see alignment E=2.5e-31 PF06429: Flg_bbr_C" amino acids 395 to 439 (45 residues), 54.9 bits, see alignment 1e-18

Best Hits

KEGG orthology group: K02390, flagellar hook protein FlgE (inferred from 100% identity to ppu:PP_4388)

Predicted SEED Role

"Flagellar hook protein FlgE" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88ER5 at UniProt or InterPro

Protein Sequence (440 amino acids)

>PP_4388 Flagellar hook protein FlgE (Pseudomonas putida KT2440)
MSFNIGLSGLYAANKALNVTGNNIANVATTGFKSSRAEFADQYSNSIRGTSAGKTVIGTG
VKTAAVSQLFTPGNINATGQALDMAIDGNGFFALNDNGSKIYTRAGAFYSDKAGYVVNAS
GANLQGYAVGEDGKIIQGILTDLQIDTSNLTPKPTSLITEKINLNSAATSPSVTPFDPTN
ASSYNFTFNTPVFDSQGNEHQMNQYFIKDDATNTWSMHTTIDGRNPANPASTTPLVNAVP
FKSDGTLDKDAMTAGDVAGGLSIGEDKIFQLNDWIPAQKNAAGEWSANGAIANADGVKLD
MLGATQYNASSAEIAKTQDGFATGELSGLSIDQNGYLFASFTNGKDKVIGQVAIANFANL
QGLTPIGGTNWKESYASGVPVIGAPDTGTLGQISGGALEDSNVDLTGELVNLIKAQSNYQ
ANAKTISTESTIMQTIIQMT