Protein Info for PP_4361 in Pseudomonas putida KT2440

Annotation: Flagellar hook-length control protein FliK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 421 PF02120: Flg_hook" amino acids 291 to 374 (84 residues), 66.9 bits, see alignment E=5.7e-23

Best Hits

KEGG orthology group: K02414, flagellar hook-length control protein FliK (inferred from 100% identity to ppu:PP_4361)

Predicted SEED Role

"Flagellar hook-length control protein FliK" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EU2 at UniProt or InterPro

Protein Sequence (421 amino acids)

>PP_4361 Flagellar hook-length control protein FliK (Pseudomonas putida KT2440)
MPVAPNPLLQANAIAKTSRSATVQADKPLQALGDKGDAFGQVMARQGRDKVTGQDDKVAQ
AKPKDKPDAAAGGKKDPAGKAAVADDGNKLPEEDSAQADTGVRDASLVAGQVTDAQPGAQ
LIQAQAEAVAPVLQAATAQTQVSAALPEEPSAEEAFDPDADPLANLPTLRLALEHSAQAK
GGTSAHALDAAQAQGEEGQAAVNTLANLVEDTDGESAESGGKAFGALLEDGLKDTKSASS
DTRIDDFANRLASLTQAATAKTANAVPATANPLHQPLPMNQNAWAEGLVNRVMYLSSQNL
KSADIQLEPAELGRLDIRVNVAADQATQVTFISGHAGVRDALDSQVHRLRELFAQQGLAQ
PDVNVADQSRGQQQQQGQAQGSNLSGVAARRAEQGGAEAVDSARPLEQQVVVGDSAVDYY
A