Protein Info for PP_4354 in Pseudomonas putida KT2440

Annotation: flagellar export apparatus subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 25 50 75 89 transmembrane" amino acids 14 to 39 (26 residues), see Phobius details amino acids 51 to 70 (20 residues), see Phobius details TIGR01402: flagellar biosynthetic protein FliQ" amino acids 1 to 88 (88 residues), 97.5 bits, see alignment E=1.9e-32 PF01313: Bac_export_3" amino acids 6 to 77 (72 residues), 97.4 bits, see alignment E=1.8e-32

Best Hits

Swiss-Prot: 43% identical to FLIQ_ECOLI: Flagellar biosynthetic protein FliQ (fliQ) from Escherichia coli (strain K12)

KEGG orthology group: K02420, flagellar biosynthetic protein FliQ (inferred from 99% identity to ppg:PputGB1_3916)

Predicted SEED Role

"Flagellar biosynthesis protein FliQ" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EU9 at UniProt or InterPro

Protein Sequence (89 amino acids)

>PP_4354 flagellar export apparatus subunit (Pseudomonas putida KT2440)
MTPEVAVDLFRDALWLTTLMVAVLVVPSLLVGLVVAMFQAATQINEQTLSFLPRLLVMLI
TLIIAGPWLVQKFMEYITTLYTNIPQLIG