Protein Info for PP_4353 in Pseudomonas putida KT2440

Annotation: Flagellar biosynthetic protein fliR

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 258 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 42 to 60 (19 residues), see Phobius details amino acids 71 to 89 (19 residues), see Phobius details amino acids 91 to 114 (24 residues), see Phobius details amino acids 120 to 141 (22 residues), see Phobius details amino acids 146 to 166 (21 residues), see Phobius details amino acids 177 to 201 (25 residues), see Phobius details amino acids 213 to 233 (21 residues), see Phobius details TIGR01400: flagellar biosynthetic protein FliR" amino acids 13 to 254 (242 residues), 246.1 bits, see alignment E=2e-77 PF01311: Bac_export_1" amino acids 14 to 245 (232 residues), 223.9 bits, see alignment E=1.1e-70

Best Hits

Swiss-Prot: 42% identical to FLIR_SALTY: Flagellar biosynthetic protein FliR (fliR) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02421, flagellar biosynthetic protein FliR (inferred from 100% identity to ppu:PP_4353)

Predicted SEED Role

"Flagellar biosynthesis protein FliR" in subsystem Flagellar motility or Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EV0 at UniProt or InterPro

Protein Sequence (258 amino acids)

>PP_4353 Flagellar biosynthetic protein fliR (Pseudomonas putida KT2440)
MLELTDTQIGTWVATFILPLFRVTAVLMTMPIFGTRMLPARIRLYVAVAVTVVIVPALPP
LPEFDPLSLRGMLLCAEQIIVGALFGLALQLLFQAFVVAGQIVAVQMGMAFASMVDPANG
VNVTVISQFMTMLVSVLFLLMNGHLVVFEVLTESFTTLPVGSALVVNHFWELAGRMGWVF
GAGLLLILPVIAALLVVNIAFGVMTRAAPQLNIFSIGFPLTLVMGMFIFWVGLADVLSHY
QALASETLQWLRELARVR