Protein Info for PP_4344 in Pseudomonas putida KT2440

Annotation: flagellar export pore protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 709 transmembrane" amino acids 21 to 40 (20 residues), see Phobius details amino acids 46 to 66 (21 residues), see Phobius details amino acids 72 to 91 (20 residues), see Phobius details amino acids 121 to 141 (21 residues), see Phobius details amino acids 209 to 229 (21 residues), see Phobius details amino acids 241 to 268 (28 residues), see Phobius details amino acids 288 to 305 (18 residues), see Phobius details amino acids 311 to 328 (18 residues), see Phobius details TIGR01398: flagellar biosynthesis protein FlhA" amino acids 21 to 705 (685 residues), 855 bits, see alignment E=1.9e-261 PF00771: FHIPEP" amino acids 31 to 697 (667 residues), 878 bits, see alignment E=2.4e-268

Best Hits

Swiss-Prot: 55% identical to FLHA_YEREN: Flagellar biosynthesis protein FlhA (flhA) from Yersinia enterocolitica

KEGG orthology group: K02400, flagellar biosynthesis protein FlhA (inferred from 100% identity to ppu:PP_4344)

Predicted SEED Role

"Flagellar biosynthesis protein FlhA" in subsystem Flagellar motility or Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EV8 at UniProt or InterPro

Protein Sequence (709 amino acids)

>PP_4344 flagellar export pore protein (Pseudomonas putida KT2440)
MDRTQLISNARNNLAGLGRGNLGVPLLLLVMLAMMMLPIPPFLLDVFFTFNIALSIVVLL
VCVYALRPLDFAAFPTILLVATLLRLALNVASTRVVMLHGQEGHGAAGKVIQAFGEVVIG
GNYVVGAVVFAILMIINFVVVTKGAGRISEVSARFTLDAMPGKQMAIDADLNAGLIDQAQ
AKHRRAEVAQEAEFYGSMDGASKFVRGDAIAGLLILFINLIGGMLIGMLQHNMSFSEAGK
VYALLTIGDGLVAQLPSLLLSTAAAIMVTRASGSEDMGKLINRQMFDSPKALAVSGALMI
VMGLVPGMPHIAFLSLGLMAAGGAYLVWKKQQQAKVKAQQEAQRQQDLLPSPQRALETKE
LGWDDVTPIDMIGLEVGYRLIPLVDRNQGGQLLARIKGVRKKLSQDLGFLMPTVHIRDNL
DLQPSAYRLTLMGVILAEAEIYPDRELAINPGQVFGTLNGIAARDPAFGLEAVWIDVGQR
SQAQSLGYTVVDASTVVATHLNQILQKHCHELIGHEEVQQLLQVLAKASPKLAEELVPGV
ISLSGLLKVLQALLSEQVPVRDIRSIAEAIANNAGKSQDTAALVAAVRVGLCRAIVQSIV
GVESELPVITLEPRLEQILLNSLQRAGQGQEDGVLLEPSMAEKLQRSLIEAAQRQEMQGQ
PAILLVAGPIRAMLSRFGRLAVPNLHVLAYQEIPDNKQVTIVATVGPNG