Protein Info for PP_4338 in Pseudomonas putida KT2440

Annotation: Chemotaxis histidine kinase CheA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 747 transmembrane" amino acids 607 to 624 (18 residues), see Phobius details PF01627: Hpt" amino acids 8 to 99 (92 residues), 64.1 bits, see alignment E=2.3e-21 PF02895: H-kinase_dim" amino acids 361 to 420 (60 residues), 56.2 bits, see alignment 7.7e-19 PF02518: HATPase_c" amino acids 468 to 607 (140 residues), 67.2 bits, see alignment E=3.3e-22 PF01584: CheW" amino acids 614 to 741 (128 residues), 96.8 bits, see alignment E=1.7e-31

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 100% identity to ppu:PP_4338)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88EW4 at UniProt or InterPro

Protein Sequence (747 amino acids)

>PP_4338 Chemotaxis histidine kinase CheA (Pseudomonas putida KT2440)
MSFGADEEILQDFLVEAGEILEQLSEQLVELESRPDDADLLNAIFRGFHTVKGGAGFLQL
NELVECCHIAENVFDVLRKGERRVDAELMDVVLEALDTVNSMFGQVRERAEVTPATPALL
AALSRLAEPGAAEAVAAPVPAPVADVVAAAPAEAEEQHQDITDSEFEQLLDSLDAIKAQA
AADEQMQGETVSGAGDEITDAEFESLLDQLHGKGQFNAEVAAPPVAVSSEVVSDEITDAE
FESLLDQLHGKGTFQAGALPAAQAPAPAAPDNSAASDEISEHEFEALLDQLHGKGKFGGD
VAAVEAPAAVQAQAPAAAKAESQPVAKPAAAPAPSPASKPAAAPRAPAPAAEKHAASEAE
TTVRVDTARLDEIMNMVGELVLVRNRLVRLGLNSGDEAMSKAVSNLDVVTADLQTAVMKT
RMQPIKKVFGRFPRLVRDLARQLKKEINLELVGEETDLDKNLVEALADPLVHLVRNAVDH
GIEMPDEREASGKARTGRVVLSAEQEGDHILLSISDDGKGMDPNILRAKAVEKGLMDKDA
AERLSESDCYNLIFAPGFSTKTEISDVSGRGVGMDVVKTKISQLNGSINIFSAKGQGSKI
VIKVPLTLAIMPTLMVMLGNQAFAFPLVNVNEIFHLDLSRTNVVDGQEVVIVRDKALPLF
YLKRWLVQGQVHEEQHEGHVVILSVGTQRIGFVVDQLVGQEEVVIKPLGKMLQGTPGMSG
ATITGDGRIALILDVPSMLKRYAARRI