Protein Info for PP_4294 in Pseudomonas putida KT2440

Annotation: conserved inner membrane protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 304 transmembrane" amino acids 73 to 100 (28 residues), see Phobius details amino acids 149 to 167 (19 residues), see Phobius details amino acids 174 to 198 (25 residues), see Phobius details amino acids 231 to 251 (21 residues), see Phobius details amino acids 271 to 294 (24 residues), see Phobius details PF05661: DUF808" amino acids 4 to 289 (286 residues), 383.1 bits, see alignment E=4.2e-119

Best Hits

Swiss-Prot: 63% identical to YEDI_ECOLI: Inner membrane protein YedI (yedI) from Escherichia coli (strain K12)

KEGG orthology group: K09781, hypothetical protein (inferred from 100% identity to ppu:PP_4294)

Predicted SEED Role

"Membrane protein, putative"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88F06 at UniProt or InterPro

Protein Sequence (304 amino acids)

>PP_4294 conserved inner membrane protein (Pseudomonas putida KT2440)
MAGSSLLVLIDDIATLLDDVSLMTKVAAKKTAGVLGDDLALNAQQVTGVRADRELPVVWA
VAKGSFVNKAILVPAALLISAFIPWAVTPLLMLGGAFLCFEGFEKLAHKFLHSKEEDEAQ
HEARKEAVADASVDLVAFEKAKIKGAVRTDFILSAEIIAITLGIVADSPLTQQIIVLSGI
AVVMTIGVYGLVGGIVKLDDLGLWMARKTSRLIQAVGNGILRAAPYMMKSLSVIGTAAMF
LVGGGILVHGIEPLHHAIEAFSEGRGGALTSALLNGGVGVLAGAVVLAVVSVAGKLWRAV
RPAN