Protein Info for PP_4236 in Pseudomonas putida KT2440

Annotation: putative Thiol:disulfide interchange protein dsbE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 278 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 44 to 65 (22 residues), see Phobius details amino acids 81 to 99 (19 residues), see Phobius details amino acids 109 to 130 (22 residues), see Phobius details PF01790: LGT" amino acids 4 to 99 (96 residues), 40.8 bits, see alignment E=4.3e-14 PF08534: Redoxin" amino acids 132 to 254 (123 residues), 65.4 bits, see alignment E=1.2e-21 PF00578: AhpC-TSA" amino acids 133 to 245 (113 residues), 63.2 bits, see alignment E=6e-21 PF13905: Thioredoxin_8" amino acids 156 to 244 (89 residues), 34.9 bits, see alignment E=4.1e-12 PF00085: Thioredoxin" amino acids 157 to 203 (47 residues), 28.6 bits, see alignment 3e-10

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_4236)

Predicted SEED Role

"Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88F63 at UniProt or InterPro

Protein Sequence (278 amino acids)

>PP_4236 putative Thiol:disulfide interchange protein dsbE (Pseudomonas putida KT2440)
MLTVTLGPLTMALNHLLMLTALVIASVVGWWVARRGGESPESALFNLFLIGLLCARAGFV
LAYWPMYQDDPLQIIDIRDGGFLFWSGLAGIVLGALWQGWRHPGLRRPLGWALFSGALFW
GLASLGGHLYSKGTELPELSLNNAGGQAVALHSYRGKPLVINIWATWCPPCRREMPVLQQ
AQSDYPHVTFLFVNQGETPENVSTFLATTGLSLTHVLFDGTGLLAQRVGSMALPTTLFYD
ANGRLVGSHLGELSRASLRHALEPFDRAIAPAPAAQGN