Protein Info for PP_4235 in Pseudomonas putida KT2440

Annotation: thiol/disulfide interchange protein 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 571 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 158 to 188 (31 residues), see Phobius details amino acids 199 to 223 (25 residues), see Phobius details amino acids 235 to 258 (24 residues), see Phobius details amino acids 279 to 308 (30 residues), see Phobius details amino acids 315 to 338 (24 residues), see Phobius details amino acids 350 to 369 (20 residues), see Phobius details amino acids 375 to 394 (20 residues), see Phobius details amino acids 405 to 424 (20 residues), see Phobius details PF11412: DsbD_N" amino acids 25 to 132 (108 residues), 94.3 bits, see alignment E=1.6e-30 PF02683: DsbD_TM" amino acids 158 to 371 (214 residues), 261.7 bits, see alignment E=1.3e-81 PF13386: DsbD_2" amino acids 164 to 360 (197 residues), 36.3 bits, see alignment E=1.7e-12 PF13899: Thioredoxin_7" amino acids 460 to 531 (72 residues), 50 bits, see alignment E=8.3e-17 PF13098: Thioredoxin_2" amino acids 468 to 562 (95 residues), 34.3 bits, see alignment E=7.9e-12 PF00085: Thioredoxin" amino acids 472 to 560 (89 residues), 26 bits, see alignment E=2.3e-09

Best Hits

KEGG orthology group: K04084, thiol:disulfide interchange protein DsbD [EC: 1.8.1.8] (inferred from 100% identity to ppu:PP_4235)

Predicted SEED Role

"Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8)" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange (EC 1.8.1.8)

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.8

Use Curated BLAST to search for 1.8.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88F64 at UniProt or InterPro

Protein Sequence (571 amino acids)

>PP_4235 thiol/disulfide interchange protein 2 (Pseudomonas putida KT2440)
MRVLLLFLTLLLAGPLQANPFDVKPDFLPVDQAFVLTHDRQADGQMRLYFQIKPGYYLYQ
KRLKFDGLPTEQHPQLPPALNHHDEFFGDSAVYRDQLELLLPANAQGQLRLGWQGCADAG
LCYPPQTTSIDLGGTVTPAAEQASDQALASGLQQGHLAWSLLAFFGLGLLLAFTPCSLPM
LPILAGLVLGNGASARRGWLLAGVYVLSMALVYAGLGVVAALLGASLQAWLQQPWLLGSL
AGLFVLLALPMFGVFELQLPAALRDRLDRAGQGTRGGNLYGAALLGALSGLLLGPCMTAP
LAGALLYIAQSGDVLQGALVLFSLGLGMGVPLLLLVTLGNRYLPRPGAWMNRVKGVFGFV
FLAMALYTVRSLLPATLLLALSGAWLIALAWAAWPALQRLPALRAVPLLGALWGGLLLVG
AAAGGDDLWQPLRPFAGGVAATAPSQHAEDRFVTVSRPEDLQRELDAAKARGQWVMLDYY
ADWCVSCKVMEKQVFARSDVQAGLAGVHLLRLDVTADSPASKALLQRYQVPGPPSIIWIG
PEGEERRARRITGEVDAAAFQQHWGQTRSQG