Protein Info for PP_4203 in Pseudomonas putida KT2440

Annotation: Electron transfer flavoprotein-ubiquinone oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 560 PF01946: Thi4" amino acids 10 to 65 (56 residues), 27.8 bits, see alignment 5e-10 PF01494: FAD_binding_3" amino acids 14 to 209 (196 residues), 23.2 bits, see alignment E=1.3e-08 PF13450: NAD_binding_8" amino acids 18 to 63 (46 residues), 29.2 bits, see alignment 3.3e-10 PF21162: ETFQO_UQ-bd" amino acids 219 to 315 (97 residues), 140.8 bits, see alignment E=5.8e-45 PF05187: Fer4_ETF_QO" amino acids 456 to 557 (102 residues), 171.5 bits, see alignment E=1.6e-54

Best Hits

Swiss-Prot: 85% identical to ETFD_PSEAE: Electron transfer flavoprotein-ubiquinone oxidoreductase (PA2953) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00311, electron-transferring-flavoprotein dehydrogenase [EC: 1.5.5.1] (inferred from 100% identity to ppu:PP_4203)

Predicted SEED Role

"Electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases (EC 1.5.5.1)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.5.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88F95 at UniProt or InterPro

Protein Sequence (560 amino acids)

>PP_4203 Electron transfer flavoprotein-ubiquinone oxidoreductase (Pseudomonas putida KT2440)
MSRREPVEREYMEFDVVIVGAGPAGLSAACRLKQKAAEAGSEISVCVVEKGSEVGAHILS
GAVFEPRALNELFPDWKELGAPLNTEVKRDDIYVLKDAGSATKVPDLFVPKTMHNHGNYI
ISLGNLCRWLAQQAENLGVEIYPGFAAQEALFDDNGVVRGIVTGDLGVDREGNPKDGLYT
PGMELRAKYTLFAEGCRGHIGKQLIKRFNLDSESDVQHYGIGLKEIWEIDPAKHEQGLVV
HTAGWPLDVVAKENTGGSFLYHLENNQVVVGLIVDLSYANPYLSPFDEFQRLKHHPVISQ
YLEGGKRISYGARALAKGGINSLPKMVFNGGALIGCDLGTMNVAKIKGSHTAMKSGMLAA
EAVADALIAGSEGGDQLNSYVSAFKASWLYEELFASRNFGPAMHKFGPLLGAAFNYVDQN
WFGGKLPFTLHDTKPDYACLKLAADSKKIDYPKPDGKLSFDKLSSVFLSSTNHEEEQPCH
LKLTDPSVPIASNLPLYDEPAQRYCPAGVYEVVTQEDGNKRFQINAQNCVHCKTCDIKDP
AQNITWVTPEGAGGPNYPNM