Protein Info for PP_4189 in Pseudomonas putida KT2440

Annotation: 2-oxoglutarate decarboxylase, thiamine-requiring E1 subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 943 PF16078: 2-oxogl_dehyd_N" amino acids 12 to 50 (39 residues), 62.5 bits, see alignment (E = 4.3e-21) TIGR00239: oxoglutarate dehydrogenase (succinyl-transferring), E1 component" amino acids 15 to 941 (927 residues), 1280.1 bits, see alignment E=0 PF00676: E1_dh" amino acids 230 to 527 (298 residues), 163.2 bits, see alignment E=1.4e-51 PF02779: Transket_pyr" amino acids 599 to 791 (193 residues), 170.1 bits, see alignment E=7.3e-54 PF16870: OxoGdeHyase_C" amino acids 797 to 941 (145 residues), 180.9 bits, see alignment E=2.7e-57

Best Hits

Swiss-Prot: 83% identical to ODO1_AZOVI: 2-oxoglutarate dehydrogenase E1 component (sucA) from Azotobacter vinelandii

KEGG orthology group: K00164, 2-oxoglutarate dehydrogenase E1 component [EC: 1.2.4.2] (inferred from 84% identity to avn:Avin_29770)

Predicted SEED Role

"2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2)" in subsystem TCA Cycle (EC 1.2.4.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.4.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FA9 at UniProt or InterPro

Protein Sequence (943 amino acids)

>PP_4189 2-oxoglutarate decarboxylase, thiamine-requiring E1 subunit (Pseudomonas putida KT2440)
MQESVMQRMWDSAHLSGGNAAYVEELYELYLHDPNAVPEEWRTYFQKLPADGSTATDVSH
STIRDHFVLLAKNQRRAQPVSAGSVSSEHEKKQVEVLRLIQAYRMRGHQAAKLDPLGLWQ
RPAPVDLSINHYGLTNADLDTTFRAGDLFIGKEEASLRDIFDALQKTYCRTIGAEFTHIV
DSEQRSWFQQRLESVRGRPEYSADVQAHLLERVTAGEGLEKYLGTKYPGTKRFGLEGGES
LIPMLDEMIQRSGSYGAKEVVIGMAHRGRLNVLVNTFGKNPRELFDEFEGKKMNELGSGD
VKYHQGFSSNVMTPGGEVHLAMAFNPSHLEIVSPVVEGSVRARQDRRNDTVGDKVVPISI
HGDAAFAGQGVVMETFQMSQTRGFKTGGTVHIVINNQVGFTISNPLDARSTEYATDVAKM
IQAPILHVNGDDPEAVLFVTQLAIDYRMQFKRDVVIDLVCYRRRGHNEADEPNGTQPLMY
QQISKQRTTRELYAEQLIQAGRIDAERAQAKIDEYRNALDNGLHVVKSLVKEPNRELFVD
WRPYLGHAWTARHDTRFDLKTLQDLSAKLLELPEGFVVQRQVSKIYEDRQKMQAGGLPIN
WGYAETMAYATLQFEGHPIRMTGQDIGRGTFSHRHAVLHNQKDASTYIPLQNLFPGQPRF
DLYDSFLSEEAVLAFEYGYSTTMPNALVIWEAQFGDFANGAQVVIDQFITSGEHKWGRLC
GLTMLLPHGYEGQGPEHSSARLERYLQLCAEHNIQVCVPTTPAQIYHLLRRQVIRPLRKP
LVVLTPKSLLRHKLAISTLEDLAEGSFQTVIPEIDAIDPAKVERLVLCSGKVYYDLLEKR
RAEGREDIAILRIEQLYPFPEDDLVEILAPYTNLKHAVWCQEEPMNQGAWYSSQHHMRRI
LGRHNKALVLEYAGRDASAAPACGYASKHAEQQEKLLQDAFTV