Protein Info for PP_4184 in Pseudomonas putida KT2440

Annotation: branched chain amino acid transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 437 transmembrane" amino acids 9 to 28 (20 residues), see Phobius details amino acids 40 to 64 (25 residues), see Phobius details amino acids 77 to 97 (21 residues), see Phobius details amino acids 118 to 138 (21 residues), see Phobius details amino acids 150 to 172 (23 residues), see Phobius details amino acids 193 to 212 (20 residues), see Phobius details amino acids 224 to 245 (22 residues), see Phobius details amino acids 280 to 302 (23 residues), see Phobius details amino acids 312 to 331 (20 residues), see Phobius details amino acids 337 to 358 (22 residues), see Phobius details amino acids 369 to 388 (20 residues), see Phobius details amino acids 408 to 426 (19 residues), see Phobius details PF05525: Branch_AA_trans" amino acids 7 to 427 (421 residues), 529.1 bits, see alignment E=4.5e-163 TIGR00796: branched-chain amino acid transport system II carrier protein" amino acids 13 to 414 (402 residues), 528.1 bits, see alignment E=8e-163

Best Hits

Swiss-Prot: 78% identical to BRAZ_PSEAE: Branched-chain amino acid transport system 3 carrier protein (braZ) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K03311, branched-chain amino acid:cation transporter, LIVCS family (inferred from 100% identity to ppf:Pput_1670)

MetaCyc: 68% identical to branched chain amino acid transporter BrnQ (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-126; TRANS-RXN-126A; TRANS-RXN-126B

Predicted SEED Role

"Branched-chain amino acid transport system carrier protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FB4 at UniProt or InterPro

Protein Sequence (437 amino acids)

>PP_4184 branched chain amino acid transporter (Pseudomonas putida KT2440)
MKVLKGQDILALGFMTFALFVGAGNIIFPPIVGLQSGPHVWMAALGFLVTAVGLPVITVV
ALAKVGGGMDALSSPIGKFFGGLLAAVCYLSVGPLFATPRTATVSFEVGVAPLTGESPLA
LFIYSLVYFIVVLAVSMYPGKLLDTVGRFLAPLKIIALAVLGIAAFALPAGSIGDAQPAY
AAAAFSKGFSDGYLTMDTLGALVFGIVIVNAIRSRGVESPRLITRYAIIAGLIAGVGLAL
VYISLFRLGATSHDIAADATNGAAVLHAYVQHTFGSLGSGFLAVLIALACLVTAVGLTCA
CAEYFSQILPLSYRALVVILAGFSLLISNLGLTKLIMFSIPVLTAIYPPCIVVVGLSFVK
DLWNSPTRILAPVMLVSLLFGMVDAIKGSSLAHVLPDFMAHLPLSDAGMAWLVPSVVTLV
GAVACDRMLGKPREALA