Protein Info for PP_4179 in Pseudomonas putida KT2440

Annotation: Chaperone protein HtpG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 634 PF02518: HATPase_c" amino acids 31 to 186 (156 residues), 32.9 bits, see alignment E=1.1e-11 PF13589: HATPase_c_3" amino acids 33 to 167 (135 residues), 39.5 bits, see alignment E=8.5e-14 PF00183: HSP90" amino acids 219 to 629 (411 residues), 438.9 bits, see alignment E=4.8e-135

Best Hits

Swiss-Prot: 100% identical to HTPG_PSEPK: Chaperone protein HtpG (htpG) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K04079, molecular chaperone HtpG (inferred from 100% identity to ppu:PP_4179)

MetaCyc: 60% identical to chaperone protein HtpG (Escherichia coli K-12 substr. MG1655)
Non-chaperonin molecular chaperone ATPase. [EC: 3.6.4.10, 5.6.1.7]

Predicted SEED Role

"Chaperone protein HtpG" in subsystem Protein chaperones

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.6.4.10 or 5.6.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FB9 at UniProt or InterPro

Protein Sequence (634 amino acids)

>PP_4179 Chaperone protein HtpG (Pseudomonas putida KT2440)
MTVETQKETLGFQTEVKQLLHLMIHSLYSNKEIFLRELISNASDAADKLRFEALAKPELF
EGDADLKIRLSFDKDAGTVTLEDNGIGMSREDVIAHLGTIAKSGTADFMKNLTGDQKKDS
HLIGQFGVGFYSAFIVADKVDVYSRRAGQPAAEGVHWSSKGEGEFEVATIDKPQRGTRIV
LHLKKDEQEFADGWRLRNVVKKYSDHIALPIQLPKEQAATEGEEQPAEEWETVNRASALW
TRSRTEVKDEEYQEFYKHIGHDFENPLAWSHNKVEGKLEYNSLLYVPARAPFDLYQREAP
RGLKLYVQRVFIMDQAESFLPLYLRFIKGVVDSNDLSLNVSREILQKDPIIDSMKTALTK
RVLDMLEKLAKNEPEKYKGFWKNFGQVLKEGPAEDFANKEKIAGLLRFASTQDDSGEQSV
ALADYLARAKEGQDKIYYLTGESYAQVKNSPHLEVFRKKGIEVLLLTDRIDEWLMSYLNE
FDGKAFVDIARGDLDLGKLDSEEDKKAQEEVAKDKEGLVERLKGALGDSVAEVRVSHRLT
DSPAILAIGEQDLGLQMRQILEASGQKVPESKPIFEFNPSHPLIEKLDHEQSEDRFAELS
HILFDQAALAAGDSLKDPAAYVRRLNKLLVELSA