Protein Info for PP_4171 in Pseudomonas putida KT2440

Annotation: conserved exported protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 704 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details PF07715: Plug" amino acids 49 to 155 (107 residues), 73.9 bits, see alignment E=1.5e-24 PF00593: TonB_dep_Rec_b-barrel" amino acids 380 to 667 (288 residues), 110.1 bits, see alignment E=2.6e-35

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 100% identity to ppu:PP_4171)

Predicted SEED Role

"Outer membrane receptor proteins, mostly Fe transport" in subsystem Hemin transport system

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FC7 at UniProt or InterPro

Protein Sequence (704 amino acids)

>PP_4171 conserved exported protein of unknown function (Pseudomonas putida KT2440)
MFPGTPPYPRLLLLTALLGGQAVADDLFIDNTDLPQVLTATRLKQSPAAVPGSMTVLDSE
LIRASGARDIPELLRLVPGMMIGYGAGNQPTVNYHGSNVNDARRMQVLIDGRSVYRAGLA
TVDWSDIPLAMEDIERIEVFRGPNTVSYGANALMAVVNILTRKPADSHGTRLKVTRGQDG
INDFYASHGLGWDGGDMRLSLSGQQDDGFDENQFGQDYRDSRRLTRFNLSANHNLAVDQT
LEWQLAAKEGSNQRPYTYQPVFQQYESGNNADVNAKDYAGSLRWTKDFSAEHSLYIQGSA
QHFDRQQVWRACDALQSFSPELTQLWQMNPDFAEKVARGIATGAPLSTDDPAMKGLLQKV
QQQWEQGGQEQVCGDVDQSTRETRYDLEIQDTLSLTDSLRLLSGMNYRYDRADSQTYFNG
SIDDQTWRLFGQLEWRADEHWILQGGAMFEDSRLSGSSLTPRVAVNYLITPRHGLRAVYS
EAVRSPDMFENNVNWSYTVKNLTPNSFGLQNGEYFVKTRGPGDLEQERMRSRELGYNGYF
SDLDLSLDVKLFYDEITGMISEPLRNNQYIASNANKARFSGSEAQMDWRPTLRDRLRLTY
AYVDAWASNPDDRRLSARNSGSAGWMREWGAGWSSALFYYGDDALNQYRYERLDLRLAKR
FRVYGSNLELAALWQQRLDDEPTSALQNRYDSRHRLSVSAELEF