Protein Info for PP_4160 in Pseudomonas putida KT2440

Annotation: K+ transporting ATPase, KdpB subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 684 transmembrane" amino acids 45 to 65 (21 residues), see Phobius details amino acids 71 to 92 (22 residues), see Phobius details amino acids 223 to 245 (23 residues), see Phobius details amino acids 256 to 280 (25 residues), see Phobius details amino acids 589 to 609 (21 residues), see Phobius details amino acids 618 to 637 (20 residues), see Phobius details amino acids 657 to 678 (22 residues), see Phobius details TIGR01497: K+-transporting ATPase, B subunit" amino acids 22 to 678 (657 residues), 957.9 bits, see alignment E=2.5e-292 TIGR01494: HAD ATPase, P-type, family IC" amino acids 79 to 348 (270 residues), 99.1 bits, see alignment E=2.1e-32 amino acids 373 to 606 (234 residues), 151.4 bits, see alignment E=2.9e-48 PF00122: E1-E2_ATPase" amino acids 115 to 288 (174 residues), 103.7 bits, see alignment E=1.7e-33 PF00702: Hydrolase" amino acids 307 to 532 (226 residues), 87.5 bits, see alignment E=3.3e-28 PF12710: HAD" amino acids 444 to 528 (85 residues), 30.6 bits, see alignment E=8.8e-11

Best Hits

Swiss-Prot: 83% identical to KDPB_PSEAE: Potassium-transporting ATPase ATP-binding subunit (kdpB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01547, K+-transporting ATPase ATPase B chain [EC: 3.6.3.12] (inferred from 99% identity to ppf:Pput_1708)

Predicted SEED Role

"Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1)" in subsystem Potassium homeostasis (EC 3.6.3.12, TC 3.A.3.7.1)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.12

Use Curated BLAST to search for 3.6.3.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A140FWL1 at UniProt or InterPro

Protein Sequence (684 amino acids)

>PP_4160 K+ transporting ATPase, KdpB subunit (Pseudomonas putida KT2440)
MNMPIPEVNSRHSAKDQTRFAALWRPALVQAFVKLDPRQLKRSPVMLVVALTAVLTTVLC
FAPGGGVSIGVAVQIALWLWFTVLFANFAEALAEGRGKARADSLKAGSQGLTAQRRLRDG
SYETVAASALRKDDVVRVVAGEMIPGDGEVIEGIAAVNEAAITGESAPVIRESGGDRSAV
TGNTRLVSDWLLIRITSNPGESTLDRMIALVEGAKRQKTPNEIALDILLIGLTLIFLIVV
VTLQPFAHFAGGSLPLIFLAALLVTLIPTTIGGLLSAIGIAGMDRLVRLNVIARSGRAVE
AAGDVHTLMLDKTGTITFGNRRCSALHAAPGITAKELGEGALLASLADDTAEGKSIVEYL
RQLHDFVDPPAGQFEAVAFSAETRLSGIDFEQHRYRKGAVDAVLAFVGMQRLEMPAALAR
EVERIAQSGGTPLLVCVDKRLLGVIHLKDVVKPGIRERFAELRKLGIRTVMVTGDNPLTA
AAIAAEAGVDDVLAEATPEKKLARIRQEQNDGRLVAMCGDGANDAPALAQADVGMAMNDG
TQAAREAANMVDLDSDPTKLLDVVQVGKELLVTRGALTTFSIANDVAKYFAILPALFAAI
YPQLGVLNLMQLASPQSAILSAIVFNALIIIVLIPLALRGVRVQAASAAHLLRRNLLIYG
LGGIVVPFAGIKLIDLLLNALNLV