Protein Info for PP_4157 in Pseudomonas putida KT2440

Annotation: two-component system DNA-binding response activator KdpE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 231 PF00072: Response_reg" amino acids 8 to 116 (109 residues), 95.8 bits, see alignment E=1.9e-31 PF00486: Trans_reg_C" amino acids 152 to 226 (75 residues), 81.8 bits, see alignment E=3.1e-27

Best Hits

Swiss-Prot: 51% identical to KDPE_ECOLI: KDP operon transcriptional regulatory protein KdpE (kdpE) from Escherichia coli (strain K12)

KEGG orthology group: K07667, two-component system, OmpR family, KDP operon response regulator KdpE (inferred from 98% identity to ppg:PputGB1_3729)

Predicted SEED Role

"sensor histidine kinase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FE0 at UniProt or InterPro

Protein Sequence (231 amino acids)

>PP_4157 two-component system DNA-binding response activator KdpE (Pseudomonas putida KT2440)
MSQSATLLVIDDEPQIRKFLRISLASQGYKVLEAATGAEGLAQAALGKPDLVVLDLGLPD
MDGQQVLRELREWSAVPVMVLSVRASEVQKVDALDGGANDYVTKPFGIQEFLARVRALLR
QVPQAGGSEVAASFGPLTVDFAFRRVTLDGVEVALTRKEYALLAILAGHPGRVITQQQLL
KDIWGPTHAEDTHYLRIVVGHLRQKLGDDPTAPRFIITEAGVGYRLVAPTA