Protein Info for PP_4081 in Pseudomonas putida KT2440

Annotation: putative type 4/6 secretion system

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 291 transmembrane" amino acids 250 to 270 (21 residues), see Phobius details TIGR03349: type IV/VI secretion system protein, DotU family" amino acids 69 to 275 (207 residues), 208.5 bits, see alignment E=5.2e-66 PF09850: DotU" amino acids 71 to 272 (202 residues), 213.6 bits, see alignment E=1.2e-67

Best Hits

KEGG orthology group: K11892, type VI secretion system protein ImpK (inferred from 100% identity to ppu:PP_4081)

Predicted SEED Role

"Outer membrane protein ImpK/VasF, OmpA/MotB domain"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FK9 at UniProt or InterPro

Protein Sequence (291 amino acids)

>PP_4081 putative type 4/6 secretion system (Pseudomonas putida KT2440)
MENSNPTLNDAKHVAETDLQSMLEKSLPNDAPAAAAAASTASAQEQKPEPAYQGYPADPE
FQLRGGCANLMLDAAAPLFGLVMRLRTLDELPNIKDVHQQVRIQIDSIREEIRQHGYEPA
QLLAYSYGLCLYIDEAVMERPWGKSSCWSHEPLLSIFHDETWGGEKIFTVITRLMQEPKR
YQDVLEFMYFALCLGLKGKYALAPKGEETLNALIHQLHGIIRELRGPTPEEVCDPYTNVA
PRNFRMSRIWPWWSPLVISAIAMAVAYGIYSYRLHLITTEVLESLNGILQQ