Protein Info for PP_4077 in Pseudomonas putida KT2440

Annotation: conserved protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 588 transmembrane" amino acids 542 to 559 (18 residues), see Phobius details PF05947: T6SS_TssF" amino acids 3 to 585 (583 residues), 518.7 bits, see alignment E=1.2e-159 TIGR03359: type VI secretion protein, TIGR03359 family" amino acids 4 to 588 (585 residues), 661 bits, see alignment E=1.1e-202

Best Hits

KEGG orthology group: K11896, type VI secretion system protein ImpG (inferred from 100% identity to ppu:PP_4077)

Predicted SEED Role

"Protein ImpG/VasA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FL3 at UniProt or InterPro

Protein Sequence (588 amino acids)

>PP_4077 conserved protein of unknown function (Pseudomonas putida KT2440)
MSLKDRFSEELRYLHELGNDFAKDNPQLARLLGKAGSDPDVERLMEAFAFLTAKLRLKLE
DDLPELTHPMLQILWPNYLRPLPSATIIRFSPRKQSLSQSQHIPKGARLFSKPVDAVPCE
FRTCTGVSLHPFEISAVSATQTLDSSVVRIGLQTLVERPLNTLGCARLDFHLSGDNRTAL
TLYLWISQYLKHVSVIMNGEVRRLPANSIGFPGFSPEEALLPYPQNVFDGYRILQEYFVF
PKRFHFFSITGLEKLWPAQACPQVGIEFHFTRQLPDTLRVGTEDFSLFCTPAVNLFKHSA
EPIDLASEAAQVELKPRSEAQSAYEIFSVDEVISTRTTTDGSTGEHLRTFRPFESFAHEI
EHVQGRTALYYRCQLEASLRGDGVTHRIAFVRADANSYIGELETASIDLTCTNRDLPLAL
GVDDINVLTEVTPPLATYTNICAPTRPYRPVLDGQLQWALISNMSLNYLSLLSVEPLKAV
IRTYDFAALHDIQQARTTGKRLDGIRELHTQPMDWLIKGQPIRGLHTQLKLDQAAFLCEG
DLYLFGCVLAHFFALYASINSFHQLEVINTTNNEHYTWPIQTGKQPLI