Protein Info for PP_4057 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function with autotransporter beta-domain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 358 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR01414: outer membrane autotransporter barrel domain" amino acids 62 to 358 (297 residues), 115.3 bits, see alignment E=1.8e-37 PF03797: Autotransporter" amino acids 109 to 335 (227 residues), 120.9 bits, see alignment E=4.2e-39

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_4057)

Predicted SEED Role

"outer membrane autotransporter"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FN2 at UniProt or InterPro

Protein Sequence (358 amino acids)

>PP_4057 conserved membrane protein of unknown function with autotransporter beta-domain (Pseudomonas putida KT2440)
MKSTSNPLRFDSIFYAVSTSLLLATPVETIAYELQGDPTSPGFLQQPVAAQLSLDPLSAS
GLSLGTLNAFSQQMSERHQQPAPDLIASQWAQFFPTSARGRALPPAQLEAPSQQLMIGPD
LFVRETAAGDVHRAGIFVGHNNLQSSFRGARPLLGDKQRKAVNLSGESLGVYWSMTHEQG
WHLDAVAMGSRIDVMGRGENGQRLDDSGHAMTFSVEGGYPIRLGGNWVIEPQAQLINQQF
FPGNQVQEETLQAFDSQPSWSGRVGAKLSGRYEVRGMPIEPYVRTNVWYDFSNADEVKLD
QVDKISSSRYSTTVELGLGLVARVTPSVALFVSADYSSDVDDNDLNGLIGSLGVRMRW