Protein Info for PP_4052 in Pseudomonas putida KT2440

Annotation: 4-alpha-glucanotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 689 TIGR00217: 4-alpha-glucanotransferase" amino acids 111 to 668 (558 residues), 347.1 bits, see alignment E=1.2e-107 PF02446: Glyco_hydro_77" amino acids 157 to 658 (502 residues), 377.8 bits, see alignment E=4.3e-117

Best Hits

KEGG orthology group: K00705, 4-alpha-glucanotransferase [EC: 2.4.1.25] (inferred from 100% identity to ppu:PP_4052)

Predicted SEED Role

"4-alpha-glucanotransferase (amylomaltase) (EC 2.4.1.25)" in subsystem Glycogen metabolism or Maltose and Maltodextrin Utilization (EC 2.4.1.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FN7 at UniProt or InterPro

Protein Sequence (689 amino acids)

>PP_4052 4-alpha-glucanotransferase (Pseudomonas putida KT2440)
MSETALHRLASSVGLSRDWIDANARPQRVSDDVLRNILEGLGHPAGDDAAVAASLRAVET
AEDSEHLPPLLTADVGQPLALSRYFSAASVAHCTLEDHTSVTLSLDDQARLPGELPIGYH
QVEIDSRRFTVAVAPTRCHSLEEKVQQPPPRCWGLAVQLYSLRRPGDGGFGDCLALEQLA
RSAAERGADALAISPIHALSAIDQEHYSPYSPSSRLLLNTLYTSPAALLGEREVRMAIEA
CGLEQQLEELEQRPLVDWPRAASARLRLLEALYQGFSHGDHPLRTDFDSFREAGGQTLEH
HCRFEVLQAQAVEHGLGADWRNWPKAWHDPYHPEVEALANAYPAKVEFRAFCQWLTERGL
QRAHEAARSNGMAVGLIADLAVGADGAGSQAWSRQDELLAGLSVGAPPDILNHVGQDWGI
CAFSPDGLKRNGYRAFIEMLRANLAHAGGLRIDHVMGLRRLWLIPQGAKPSEGAYLDYPL
DDLLRLLALESVRHQAIILGEDLGTVPDSLREKLAEKAVLGMRVLPFEQTQPGQFKPILD
WPDNALATTGTHDLAPLAGWLANRDIDWRHRLQLIDAATELHWRHERQKEREGLRRILEA
NYGELKDNDALIDAAIRFVGHTRAPLVLVPLEDLLGCDEQPNLPGTTNGHPNWRRRFSRP
VRELLDDEDAARRLELLAQAREQAWERDR