Protein Info for PP_4035 in Pseudomonas putida KT2440

Annotation: putative PydP transporter, NCS1 nucleoside transporter family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 transmembrane" amino acids 49 to 73 (25 residues), see Phobius details amino acids 79 to 99 (21 residues), see Phobius details amino acids 123 to 123 (1 residues), see Phobius details amino acids 125 to 151 (27 residues), see Phobius details amino acids 161 to 182 (22 residues), see Phobius details amino acids 194 to 212 (19 residues), see Phobius details amino acids 232 to 252 (21 residues), see Phobius details amino acids 272 to 295 (24 residues), see Phobius details amino acids 315 to 337 (23 residues), see Phobius details amino acids 357 to 374 (18 residues), see Phobius details amino acids 394 to 415 (22 residues), see Phobius details amino acids 435 to 454 (20 residues), see Phobius details amino acids 465 to 485 (21 residues), see Phobius details PF02133: Transp_cyt_pur" amino acids 38 to 465 (428 residues), 308.5 bits, see alignment E=3.8e-96

Best Hits

KEGG orthology group: K03457, nucleobase:cation symporter-1, NCS1 family (inferred from 100% identity to ppf:Pput_1803)

Predicted SEED Role

"Possible pyrimidine permease in reductive pathway" in subsystem Pyrimidine utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FQ2 at UniProt or InterPro

Protein Sequence (496 amino acids)

>PP_4035 putative PydP transporter, NCS1 nucleoside transporter family (Pseudomonas putida KT2440)
MQQSRSEVVEQDGLFELSEGSDVLDSPRYNPDIAPTKVHQRTWNKWHITALWVGMSICVP
TYTLGGVLTAYFGLSVGEALLAILLANVIVLIPLTLNAFPGTKYGIPFPVLLRSSFGILG
SNVPCLIRAVVACGWFGIQTMFGGLAIHLFLGSVFDGWKALGGTGEVIGFMIFWCLNLWV
VLRGAESIKWLETLSAPLLVAVGVGLLFWALPHMSMTELLAQPPKRPEGASVVSYFCAGL
TAMVGFWATLSLNIPDFSRYARSQKDQILGQIFGLPLTMFLFAALGVVLTAASASLVGET
VSDPVSLIGKIQSPFWVALAMALIVIATLSTNTAANIVSPTNDFQNIAPRLIGRSRAVWL
TGFIGLALMGHELLKKLGLIVSDLSLESVYSNWLLGYSSLLGPIAGIMVVDYFLIRRQKL
DLAGLYRDDVYPAWNWAGFAAFALPVGLTVMAIGNSSFSWFYDYGWFTGSLLGGALYAAL
GGLAARNPVRLAKPLP