Protein Info for PP_4031 in Pseudomonas putida KT2440

Annotation: Na(+)/H(+) antiporter NhaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 transmembrane" amino acids 20 to 43 (24 residues), see Phobius details amino acids 45 to 78 (34 residues), see Phobius details amino acids 93 to 117 (25 residues), see Phobius details amino acids 125 to 143 (19 residues), see Phobius details amino acids 148 to 166 (19 residues), see Phobius details amino acids 201 to 223 (23 residues), see Phobius details amino acids 243 to 263 (21 residues), see Phobius details amino acids 300 to 319 (20 residues), see Phobius details amino acids 325 to 341 (17 residues), see Phobius details amino acids 353 to 373 (21 residues), see Phobius details amino acids 396 to 416 (21 residues), see Phobius details amino acids 454 to 472 (19 residues), see Phobius details amino acids 478 to 498 (21 residues), see Phobius details PF06450: NhaB" amino acids 6 to 498 (493 residues), 737.3 bits, see alignment E=1.1e-225 PF03600: CitMHS" amino acids 71 to 332 (262 residues), 55.6 bits, see alignment E=4.9e-19

Best Hits

Swiss-Prot: 100% identical to NHAB_PSEPK: Na(+)/H(+) antiporter NhaB (nhaB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03314, Na+:H+ antiporter, NhaB family (inferred from 100% identity to ppu:PP_4031)

MetaCyc: 60% identical to Na+:H+ antiporter NhaB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-130

Predicted SEED Role

"Na+/H+ antiporter NhaB" in subsystem ZZ gjo need homes

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FQ6 at UniProt or InterPro

Protein Sequence (500 amino acids)

>PP_4031 Na(+)/H(+) antiporter NhaB (Pseudomonas putida KT2440)
MSRPLTGALAHGFLGKSPIWYKVVICLFLVLNPLLLVTIGPVATGWALVLEFIFTLAMAL
KCYPLMPGGLLLIEALLLQMTTPQALYEELQHNFPVILLLMFMVAGIHFMKELLLFLFSR
ILLGVRSKAILALLFCVLSAFLSAFLDALTVTAVIISAAVGFYAVYHRVASGANPREDSA
LESDQQVAHLHREDLDQFRAFLRSLLMHGAVGTALGGVCTLVGEPQNLLIGHEMGWHFVD
FFLKVAPVSLPVLGAGLVTCVLLEKVRLFGYGTLMPEPVRRVLTAYAAEDDAARTQAQRI
ALWVQGLAALILIICLGLHVAEVGLIGLMVIVLITAFTGITDEHRLGRAFQDAMPFTSLL
VVFFAVVAVIHQQQLFSPLINWVLALPAEQQPGMLYLANGLLSAISDNVFVATIYITEVK
QAFLDGSMSREHFETLAVAINTGTNLPSVATPNGQAAFLFLLTSAIAPLIRLSYGRMVWM
ALPYTVVMGGLGWWAVTYWL