Protein Info for PP_4003 in Pseudomonas putida KT2440

Annotation: Outer-membrane lipoprotein carrier protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 228 signal peptide" amino acids 1 to 43 (43 residues), see Phobius details TIGR00547: outer membrane lipoprotein carrier protein LolA" amino acids 30 to 225 (196 residues), 98.5 bits, see alignment E=1.8e-32 PF03548: LolA" amino acids 56 to 218 (163 residues), 190.9 bits, see alignment E=1.4e-60

Best Hits

Swiss-Prot: 100% identical to LOLA_PSEP1: Outer-membrane lipoprotein carrier protein (lolA) from Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1)

KEGG orthology group: K03634, outer membrane lipoprotein carrier protein (inferred from 100% identity to ppu:PP_4003)

Predicted SEED Role

"Outer membrane lipoprotein carrier protein LolA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FS9 at UniProt or InterPro

Protein Sequence (228 amino acids)

>PP_4003 Outer-membrane lipoprotein carrier protein (Pseudomonas putida KT2440)
MNTLPGANGGARPFQRSMRIPMRAIRMLLVSALTLGSVTAYAGEQDVQRLTQLLEKSQTI
EANFSQLTLGADGTSLQETSGKMTVKRPGLFYWHTDAPQEQVVVSDGKNVTLWDPDLEQA
TIKKLDVRLNQTPALLLSGDVSKISQSFDIASKEQGEVMDFTLKPKTKDTLFDSLRVSFR
KGLINDMQLIDSVGQRTNILFNGVKANQAVPDSKFKFDIPKGADVIKE