Protein Info for PP_3958 in Pseudomonas putida KT2440

Annotation: Na(+)/H(+) antiporter NhaA 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 453 transmembrane" amino acids 23 to 45 (23 residues), see Phobius details amino acids 74 to 92 (19 residues), see Phobius details amino acids 111 to 129 (19 residues), see Phobius details amino acids 140 to 159 (20 residues), see Phobius details amino acids 168 to 189 (22 residues), see Phobius details amino acids 195 to 212 (18 residues), see Phobius details amino acids 219 to 251 (33 residues), see Phobius details amino acids 313 to 334 (22 residues), see Phobius details amino acids 345 to 370 (26 residues), see Phobius details amino acids 385 to 408 (24 residues), see Phobius details amino acids 419 to 438 (20 residues), see Phobius details PF06965: Na_H_antiport_1" amino acids 22 to 434 (413 residues), 467.4 bits, see alignment E=1.6e-144 TIGR00773: Na+/H+ antiporter NhaA" amino acids 23 to 435 (413 residues), 396.4 bits, see alignment E=6.4e-123

Best Hits

Swiss-Prot: 100% identical to NHAA2_PSEPK: Na(+)/H(+) antiporter NhaA 2 (nhaA2) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K03313, Na+:H+ antiporter, NhaA family (inferred from 100% identity to ppu:PP_3958)

Predicted SEED Role

"Na+/H+ antiporter NhaA type" in subsystem Na(+) H(+) antiporter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FW9 at UniProt or InterPro

Protein Sequence (453 amino acids)

>PP_3958 Na(+)/H(+) antiporter NhaA 2 (Pseudomonas putida KT2440)
MHKQELPRAQKLAERAFANLERFLHIEAVSGIVLLIAAVAALIWANSPAADSYEALWHTP
LTFGVGSHVYSQSLHFWINDGLMTIFFLVVGMEIRREIHEGALSSLRQATLPMAAAVGGV
AVPALLYLSFGHAPADQQGWAVPTATDIAFAVGVLALLGKSIPSNVRVFLLALAIIDDII
AVLIIAFFYSGGLDYTGFGVAVIGLLMVIGLQKIGVGSAYAYVIPGAIVWLGILLTGAHP
TLAGVVLGLMTPVTAMPMRERPLDAITRFTGELLGRAKAPEQDASDLMDPLKRLRLAQRE
LLPPVVRMQGTLHPWVAFGIMPVFALANAGVSLSGVDLSAEGPQWVMIAVAVALVAGKPL
GIVSVSWLMVRLGWCVLPAEVNWRSIMLVGLLAGIGFTMSIFIANLAFVDPGSLGAAKLG
VLSASLIAAVLGLTWGVWSMRSAASATKAGSPT