Protein Info for PP_3941 in Pseudomonas putida KT2440

Annotation: maleamate amidohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00857: Isochorismatase
  • amino acids 26 to 203 (178 residues), 123.4 bits, E=5.8e-40

Best Hits

Swiss-Prot: 100% identical to NICF_PSEPK: Maleamate amidohydrolase (nicF) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K13995, maleamate amidohydrolase [EC: 3.5.1.107] (inferred from 100% identity to ppu:PP_3941)

MetaCyc: 100% identical to maleamate amidohydrolase (Pseudomonas putida KT2440)
RXN-646 [EC: 3.5.1.107]

Predicted SEED Role

"N-carbamoylsarcosine amidase (EC 3.5.1.59)" (EC 3.5.1.59)

MetaCyc Pathways

• nicotinate degradation I (6/6 steps found)
• creatinine degradation II (3/5 steps found)
• picolinate degradation (4/7 steps found)
• nicotinate degradation II (2/5 steps found)
• nicotine degradation II (pyrrolidine pathway) (6/11 steps found)
• nicotine degradation III (VPP pathway) (5/10 steps found)
• superpathway of nicotinate degradation (6/18 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.107 or 3.5.1.59

Sequence Analysis Tools

See Q88FY5 at UniProt or InterPro

Protein Sequence (213 amino acids)

>PP_3941 maleamate amidohydrolase (Pseudomonas putida KT2440)
MSDAQSARDNYQGVWGQRIGFGRKPALLMIDFMQGYTTPGAPLYAPGVVAAVEQAAGLLA
LARDCGTLVVHTNIRYQPPHFADGGVWVRKAPVMKDMVEGNPLAAFCEAVAPQAGEVVLS
KQYASAFFATSLAPLLHAQGVDTVVLAGCSTSGCIRASAVDAMQHGFRTIVVRECVGDRH
SDPHEANLFDIDSKYGDVVTRQDAMQQLRHLAG