Protein Info for PP_3940 in Pseudomonas putida KT2440

Annotation: Putative metabolite transport protein NicT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 437 transmembrane" amino acids 24 to 41 (18 residues), see Phobius details amino acids 61 to 81 (21 residues), see Phobius details amino acids 93 to 113 (21 residues), see Phobius details amino acids 119 to 140 (22 residues), see Phobius details amino acids 152 to 175 (24 residues), see Phobius details amino acids 187 to 209 (23 residues), see Phobius details amino acids 254 to 275 (22 residues), see Phobius details amino acids 288 to 308 (21 residues), see Phobius details amino acids 320 to 339 (20 residues), see Phobius details amino acids 345 to 367 (23 residues), see Phobius details amino acids 377 to 399 (23 residues), see Phobius details amino acids 411 to 432 (22 residues), see Phobius details PF07690: MFS_1" amino acids 32 to 394 (363 residues), 151.6 bits, see alignment E=1.4e-48

Best Hits

Swiss-Prot: 100% identical to NICT_PSEPK: Putative metabolite transport protein NicT (nicT) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: None (inferred from 100% identity to ppf:Pput_1897)

Predicted SEED Role

"Nitrate/nitrite transporter" in subsystem Nitrate and nitrite ammonification

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88FY6 at UniProt or InterPro

Protein Sequence (437 amino acids)

>PP_3940 Putative metabolite transport protein NicT (Pseudomonas putida KT2440)
MPIANATTVHSDIDHGTKALYSKITWRLIPFLCFCYLAAYLDRINVGFAKLQMLEDLQFS
TAAYGLGAGLFFVGYIIFEVPSNLILQRVGAKLWIARIMITWGLLSACTMFVTSTTQFYI
LRFLLGAAEAGFLPGVLYYLTMWYPTYRRGRIIALFMIGLPLSSVIGGPISGWIMGHFDQ
VQGLHGWQWLFLLEAIPSVLLGILTFWALPNHFQQAKWLSADDKAQLAADLAADDAEGKD
SKHSFRDGFFNLKVWMLGGIDFSILLSAYAMGFWMPTFIRDAGVSDTFHIGLLTAIPSLA
ALAGMLMIGASSDRHRERRWHIIVPFIIGAIAMASSTLFSQNLVMTVVLFAIASAAIIGA
VPVFFSLPATFLKGTAAATGFALACSVANIAGLVSNSLMGVVTDLTGTSHAALWVFAGCL
ILSCFLVIALPAKLVNR