Protein Info for PP_3893 in Pseudomonas putida KT2440

Annotation: putative Phage DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 465 PF00772: DnaB" amino acids 9 to 105 (97 residues), 60.5 bits, see alignment E=2.9e-20 TIGR03600: phage replicative helicase, DnaB family" amino acids 10 to 424 (415 residues), 527.7 bits, see alignment E=1.2e-162 PF13481: AAA_25" amino acids 176 to 358 (183 residues), 32.1 bits, see alignment E=1.8e-11 PF03796: DnaB_C" amino acids 177 to 433 (257 residues), 260.2 bits, see alignment E=4e-81

Best Hits

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 100% identity to ppu:PP_3893)

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88G33 at UniProt or InterPro

Protein Sequence (465 amino acids)

>PP_3893 putative Phage DNA helicase (Pseudomonas putida KT2440)
MIATRPLVAEEAEFGVIGSLMHQPSLIETIGAKISPMHFHQQDAAELFSMILAARSAGRP
ADPVSLADIRPTLSNGEMTLVRAAEIMRNTVSVANANEYARIVVERSKARVVNDLGQTII
NLASQARPIAQIIAEVQEAALSLNSEDDEPDVITLAEALGPVIDEMDDRFNGRGINGLST
GLPDLDDLIQGLRGSHVIIVAGRPGTGKTTLGLGIAEYLTIRESKSALVFSLEMAGKELA
KRSLASASSVTTGNIDTGKAMADGEKIQKITAAVGRMRDADLRICQKGGLPLSRIRNIAR
FQHKAKPLDLIVIDYIGLIAPEPGSRQQNRNLELGAISRGIKAMAKELDVPVIVLAQLNR
GIETRTAKKPQMSDLRDSGEIEQDADIILIAHRDADSDLGQSGVTEIDVVKHRHAPVGHC
LLQHQGEFARFANYAGAREQQQAAAQPARRSSRSMLNNFQPGERF