Protein Info for PP_3849 in Pseudomonas putida KT2440

Annotation: putative calcium-binding protein, hemolysin-type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 656 PF00353: HemolysinCabind" amino acids 239 to 267 (29 residues), 24.2 bits, see alignment (E = 1.4e-09) amino acids 243 to 277 (35 residues), 32.6 bits, see alignment (E = 3.1e-12) amino acids 270 to 303 (34 residues), 29.1 bits, see alignment (E = 3.9e-11) amino acids 342 to 376 (35 residues), 30.1 bits, see alignment (E = 1.9e-11) amino acids 425 to 458 (34 residues), 32 bits, see alignment (E = 4.8e-12) amino acids 506 to 541 (36 residues), 30.8 bits, see alignment 1.1e-11 amino acids 534 to 559 (26 residues), 27.2 bits, see alignment (E = 1.5e-10)

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3849)

Predicted SEED Role

"Hemolysin-type calcium-binding region"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88G76 at UniProt or InterPro

Protein Sequence (656 amino acids)

>PP_3849 putative calcium-binding protein, hemolysin-type (Pseudomonas putida KT2440)
MSGIKNIVVSFDKKFAYSYSLDDSYAEHANILVNGGVTDSWSDGSSSQTWFIADTNPAGT
YNITSINIQDLQGNIRSYSPAELEKLGINTSVDFVNSTPDTSPPSLKFLKIPTVVDLSSG
KAELTIDGIATDDLAGIKNIIVTFNKNLAYSYSSTDTYAENTSILVNGGVYDSWKDGSSS
QTWYVAGTNPSGLYNVTSVLIQDLQGNTRSYAPSELAALGINTTILMVATKSVLTSAGDS
FSGSSRADWIVGGDGNDELKGLSGDDMLEGGDGDDILDGGTGADIMIGGQGNDTYYVDWG
NDKVIETSSAGGIDTVISSVSRTLGAYQENLVLTGSSAINGTGNNLANTLTGNDADNVLN
GGAGADIMVGGLGNDTYYVDWGNDKVIETSANGGIDTIISSVSRTLGDYQENLVLTGTAA
LYGNGNNLANTLTGNNGDNVLNGGAGADTMIGGLGNDTYYVDWGNDKVIETSANGGIDTV
ISSVSRTLGDHQENLVLTGTKANYGTGNSLDNTLTGNGADNLLNGGAGNDTLVGGAGNDR
LVGDLGKDVLTGGAGNDVFAFNTTKESGLTSTAWDVITDFKRGSDKIDVSGIDANTTTNT
NEAFNVVLSGAKTFTAAGQLNFVDGVLYGNTDSDIEPEFAIQIVGVTYLASSDLIL