Protein Info for PP_3834 in Pseudomonas putida KT2440

Annotation: conserved protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 481 PF06745: ATPase" amino acids 10 to 225 (216 residues), 81.7 bits, see alignment E=2.2e-26 amino acids 244 to 460 (217 residues), 105.3 bits, see alignment E=1.3e-33 PF13481: AAA_25" amino acids 14 to 161 (148 residues), 29 bits, see alignment E=3.1e-10

Best Hits

KEGG orthology group: K08482, circadian clock protein KaiC (inferred from 100% identity to ppu:PP_3834)

Predicted SEED Role

"Circadian clock protein KaiC" in subsystem Cyanobacterial Circadian Clock

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88G91 at UniProt or InterPro

Protein Sequence (481 amino acids)

>PP_3834 conserved protein of unknown function (Pseudomonas putida KT2440)
MAQALKRLVTGIDGLDALLKGGLIAGASYIIQGPPGAGKTILANQLACGHVRGGGRVLVA
TLLSESHERLFQYLSTLDFFDPALVGDKIQFVSAFDTLEQDGLDAVVRLLRQEIARQQAS
LLIVDGVLNARVRAETALDTKKFVSELQGHAAFAGCTVLLLTSARLDEGSPEHTMVDGVI
ELGEQLVGSRAVRHVQLRKTRGSGALSGRHECLIDESGMQVYPRLEALFSHPSQLGSGTL
ARISSGVPTFDEMLGGGLATGSVSLLMGPSGIGKTSLGLAFLGASTPQAPGLHFGFYETP
ERLRSKAASLGYDFAAMEKGGSLQLCWQPTTEGLLDQVGARLLERVAAQGSKRVVIDSLG
AFSRLAIDPTRLNAFFRALAGELRARDVSVMLTWEMRDIFGAEISAPAPDLSSIVDNLML
MRFVELDSQLRRMLSVLKVRDSHHDPALHELLIVPQGITLRKAFEGACGVLSGTPTPQES
G