Protein Info for PP_3819 in Pseudomonas putida KT2440

Annotation: glutathione reductase (NADPH)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 451 TIGR01424: glutathione-disulfide reductase" amino acids 3 to 447 (445 residues), 630 bits, see alignment E=9.2e-194 PF07992: Pyr_redox_2" amino acids 5 to 317 (313 residues), 253 bits, see alignment E=1.1e-78 PF13738: Pyr_redox_3" amino acids 120 to 294 (175 residues), 61.7 bits, see alignment E=1.8e-20 PF00070: Pyr_redox" amino acids 168 to 244 (77 residues), 67.8 bits, see alignment E=2.5e-22 PF02852: Pyr_redox_dim" amino acids 338 to 446 (109 residues), 131.9 bits, see alignment E=2.9e-42

Best Hits

Swiss-Prot: 81% identical to GSHR_PSEAE: Glutathione reductase (gor) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K00383, glutathione reductase (NADPH) [EC: 1.8.1.7] (inferred from 100% identity to ppu:PP_3819)

MetaCyc: 47% identical to glutathione reductase (NADPH) (Escherichia coli K-12 substr. MG1655)
Glutathione-disulfide reductase. [EC: 1.8.1.7]

Predicted SEED Role

"Glutathione reductase (EC 1.8.1.7)" in subsystem Glutathione: Redox cycle (EC 1.8.1.7)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.1.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GA5 at UniProt or InterPro

Protein Sequence (451 amino acids)

>PP_3819 glutathione reductase (NADPH) (Pseudomonas putida KT2440)
MAFDFDLFVIGAGSGGVRAARFAAGFGAKVAVAESRYLGGTCVNVGCVPKKLLVYGAHVA
DELEQAAGFGWTLEEGHFDWGTLIANKNREIERLNGIYRNLLVNSGVTLLQGHARLTGAN
EVEVDGQRYTAEHILIATGGWPQVPDIPGKELAITSNEAFYLKDLPRRVLVVGGGYIAVE
FAGIFQGLGADTTLLYRGDLFLRGFDGSVRTHLKQELEKRGLDLQFNADIQRIDKLEDGR
LKATLKDGRELVADCVFYATGRRPMLDNLGLENTGVELDERGYIRVDEQYQTTAPSILAI
GDVIGRVQLTPVALAEGMAVARRLFKPEQYRPVDYQNIPTAVFSQPPIGTVGLTEEQALE
AGHKVQVFESRFRAMKLTLTDIQEKTLMKLVVDAETDKVLGCHMVGPDAGEIIQGLGIAL
KAGATKQQFDETIGVHPTAAEEFVTMRTVTR