Protein Info for PP_3783 in Pseudomonas putida KT2440

Annotation: syringomycin biosynthesis enzyme 2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 311 TIGR01762: chlorinating enzyme" amino acids 9 to 297 (289 residues), 357.7 bits, see alignment E=2.3e-111 PF05721: PhyH" amino acids 17 to 242 (226 residues), 97.7 bits, see alignment E=6.3e-32

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3783)

MetaCyc: 52% identical to L-threonyl-[L-threonyl-carrier protein] 4-chlorinase (Pseudomonas syringae pv. syringae)
RXN-19538 [EC: 1.14.20.15]

Predicted SEED Role

"CmaB"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.14.20.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GE1 at UniProt or InterPro

Protein Sequence (311 amino acids)

>PP_3783 syringomycin biosynthesis enzyme 2 (Pseudomonas putida KT2440)
MTNDLHQPLTDEQVAFFQKEGYLGPFPAYSQSEIEDIWRRARLKTFDHSQSVFGKAESTK
DPISSYDRHLDIDELADHVCHPAIISKLQQLLGRDVACWRSEFFPKYPGDEGTDWHQGDT
FAGLNGGKDALRWPEGSLQGGTVTVWTALSDVTLDMGPMGIIPGTQHARFYDESKAAAYS
QDKHGLVKNGVARGFYGYDYRDLQVDPDWAPDESKAIYFTMKKGEFIIFWSTLLHGSLPH
LGETTTPRLGYVSRYVPTSVRVYPDADTLTEFGRTVSLEQYGTVIVGGEDAFGHNRVRSQ
TTRGKAFNRIP