Protein Info for PP_3741 in Pseudomonas putida KT2440

Annotation: transpeptidase (penicillin-binding protein 2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 631 transmembrane" amino acids 21 to 41 (21 residues), see Phobius details TIGR03423: penicillin-binding protein 2" amino acids 19 to 609 (591 residues), 751.3 bits, see alignment E=3.9e-230 PF03717: PBP_dimer" amino acids 64 to 236 (173 residues), 156.2 bits, see alignment E=1.3e-49 PF00905: Transpeptidase" amino acids 269 to 605 (337 residues), 237.2 bits, see alignment E=2.5e-74

Best Hits

KEGG orthology group: K05515, penicillin-binding protein 2 (inferred from 100% identity to ppu:PP_3741)

Predicted SEED Role

"Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)" in subsystem Bacterial Cell Division or Bacterial Cytoskeleton or Flagellum in Campylobacter or Peptidoglycan Biosynthesis (EC 2.4.1.129)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129

Use Curated BLAST to search for 2.4.1.129

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GI2 at UniProt or InterPro

Protein Sequence (631 amino acids)

>PP_3741 transpeptidase (penicillin-binding protein 2) (Pseudomonas putida KT2440)
MPQPIPLKDHEKEKHLVNRRLLACAAMVFSLCAVLVGRLYVLQVLQHDQQTAVSENNRVH
VLPIAPERGLIYDRNGVVLADNKPSFDLTMTRERAGGDSAKVLDTLTQVLGLTEDDRKQF
DKDLRRGRKPFEPVTLMVGLSEEQIALVAVNQFRLPGLEVEPQFIREYPLAGHFAHSVGY
VGRINEKEAKTLDSTEYRGTQSIGKTGIERFYESQLHGHVGYEEVETNAQGRVMRVLNHK
DPTPGQDIVLTLDAHLQIAAEKALGDRRGSVVVLDPANGDVLAMVSNPSFDPNLFVKGIS
FKQYAALRDSIDRPLFNRVLRGLYAPGSTVKPEVAIAGLDSGVITPGSRVFDPGYYELPN
YDHKYRNWNRSGDGWVDMYTAIMRSNDTYFYDLAHKLGIDRLHDYMAEFGLGQKVSLDMF
EEASGLMPSAEWKRATRRQAWFPGETLILGIGQGYMQVTPLQLAQATSLLASKGVWHRPH
LAMTVGGDTPIDPNPMPDIVLHDKHAWDQVSQGMQMVMHDPRGIARAAAAGAQYRIAGKS
GTAQVVAIKQGERYNRNKTLERHRDNALFVGFAPAEHPSVVVAVMIENGEAGGRVAGPVV
REVMDAYLLDEQGHLKPEYAGGAPTRGVPHT