Protein Info for PP_3727 in Pseudomonas putida KT2440

Annotation: Amino-acid permease RocE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 468 transmembrane" amino acids 20 to 40 (21 residues), see Phobius details amino acids 46 to 67 (22 residues), see Phobius details amino acids 86 to 107 (22 residues), see Phobius details amino acids 127 to 147 (21 residues), see Phobius details amino acids 159 to 180 (22 residues), see Phobius details amino acids 199 to 218 (20 residues), see Phobius details amino acids 244 to 263 (20 residues), see Phobius details amino acids 290 to 315 (26 residues), see Phobius details amino acids 335 to 356 (22 residues), see Phobius details amino acids 362 to 385 (24 residues), see Phobius details amino acids 407 to 428 (22 residues), see Phobius details amino acids 434 to 453 (20 residues), see Phobius details PF00324: AA_permease" amino acids 18 to 449 (432 residues), 432.7 bits, see alignment E=1.7e-133 PF13520: AA_permease_2" amino acids 19 to 444 (426 residues), 136.9 bits, see alignment E=9.6e-44

Best Hits

Swiss-Prot: 54% identical to ROCE_BACSU: Amino-acid permease RocE (rocE) from Bacillus subtilis (strain 168)

KEGG orthology group: K02205, arginine/ornithine permease (inferred from 100% identity to ppu:PP_3727)

MetaCyc: 49% identical to CP4-6 prophage; S-methyl-L-methionine transporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-486

Predicted SEED Role

"Arginine permease RocE" in subsystem Arginine and Ornithine Degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GJ5 at UniProt or InterPro

Protein Sequence (468 amino acids)

>PP_3727 Amino-acid permease RocE (Pseudomonas putida KT2440)
MTDSVEKIQLTRALKSRHIFMLSLGGVIGTGLFMGSGVTINQGGPVGAILAYLVAGLLMY
LVMVCLGELSVQMPVSGSFQAHATKYIGPATGFMIGWVYWMSWATTVGLEFTAAGMLMTR
WFPEVPIWYWSALFVVVLFGLNALATRAFGEAEYWFSGIKVATILGFIVIGLLVIFGAIP
LSSGAPAPMLNNLVGESLFPHGLSAVFAVMMTVVYAFQGCEIMGVAAGETEHPEKSIPRA
VRNVVFRVLIFYVLAIAVLSAIVPHEQAGLMESPFVQVFDMVGIPYAADLMNFVILTAIL
SVGNSGLYASTRILWAMSKTGMAPKSLSPLSKRGVPLRALSITLCFALISLMTSFVAADT
LFMVLMAVSGMSGTVTWIVIALAQYRFRKAWLRDGGQLQDLKYKAPLYPLVPLLCITLCS
SLFVFLALDETQRPSLYWGFGFIALCYAAYFFVNRRRQGAFAPSTPGV