Protein Info for PP_3724 in Pseudomonas putida KT2440

Annotation: putative Acyl-CoA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 705 PF13380: CoA_binding_2" amino acids 26 to 133 (108 residues), 44 bits, see alignment E=4.1e-15 PF13607: Succ_CoA_lig" amino acids 155 to 288 (134 residues), 115.8 bits, see alignment E=1.9e-37 PF13549: ATP-grasp_5" amino acids 475 to 696 (222 residues), 191.2 bits, see alignment E=2.5e-60

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3724)

Predicted SEED Role

"Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GJ8 at UniProt or InterPro

Protein Sequence (705 amino acids)

>PP_3724 putative Acyl-CoA synthetase (Pseudomonas putida KT2440)
MSQSIRDNLKRLLAPRHLAFVGGRSMARALKRCAEGGFAGELWLVNPQHESLEGIPCVAR
VADLPYAPDAVFIATNRELTLQCVAELAARGAGGAICYASGFAESGEEGCQLQQRLLDVA
GNMALLGPNCYGLLDYLHGAALWPVAHGGQQVEKGVAILTQSGNFAYNLSMSDRSLPVAY
MASVGNQAQLGVAELMDVLLDDPRVTAIGLHLEGLKNVPGFARAACKALQQGTPIIALKT
GVSQIGAELALSHTSSLSGSDALYDSLFQRLGVIRVSGPVSFVETLKAAACGRLPADGEL
IALACSGGDAGLIADYAERNQLQLPKLEQGQVAALAEVLPGYANLVNPLDFTTAIWGDEA
ALQRMLDNTLSGAAGAAMLVLDYPAAFTGERKECDLLLGLYCDAVERHGKIGFVTSAFPE
LLPASARERLHARGIAALQGVEDGLAAWGRIVAYQKNRQRLMDQGEAVRVPLCPQALTGE
SRLLDEWQSKQALRAFGLPVPAGVLSTPERAVADAARVGYPLVLKAVSAQLPHKTEAGAV
ALNLRDARALEAALVQMRQCIADYAPQVAFDQVLLEPMAEPPLAELIVGIKRENNFALAL
VIGAGGVLVELLKDSVSLLLPTTDSAIRAALLNLRSASLLQGFRGRPAANLDALVSAIRA
VADYACENAGQLMELDVNPLMVGANGTTAVDALIRLGQAQGERHE