Protein Info for PP_3662 in Pseudomonas putida KT2440

Annotation: conserved protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 PF14793: DUF4478" amino acids 10 to 116 (107 residues), 145 bits, see alignment E=1.2e-46 PF03641: Lysine_decarbox" amino acids 208 to 331 (124 residues), 87.3 bits, see alignment E=1.6e-28 PF11892: PpnN_C" amino acids 336 to 455 (120 residues), 181.2 bits, see alignment E=1.1e-57

Best Hits

Swiss-Prot: 55% identical to PPNN_ECOL6: Pyrimidine/purine nucleotide 5'-monophosphate nucleosidase (ppnN) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K06966, (no description) (inferred from 100% identity to ppu:PP_3662)

MetaCyc: 55% identical to nucleotide 5'-monophosphate nucleosidase (Escherichia coli K-12 substr. MG1655)
AMP nucleosidase. [EC: 3.2.2.4]; Inosinate nucleosidase. [EC: 3.2.2.4, 3.2.2.12]; Pyrimidine-5'-nucleotide nucleosidase. [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]; 3.2.2.- [EC: 3.2.2.4, 3.2.2.12, 3.2.2.10]

Predicted SEED Role

"Decarboxylase family protein"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.4

Use Curated BLAST to search for 3.2.2.10 or 3.2.2.12 or 3.2.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GQ6 at UniProt or InterPro

Protein Sequence (457 amino acids)

>PP_3662 conserved protein of unknown function (Pseudomonas putida KT2440)
MPQRHVINASVSPKGSLETLSQREVQQLSEVGTGSLYTLFRQCALAILNTGAHVDNAKTI
LEAYKDFEVRIHQQDRGVRLELLNAPADAFVDGEMIASTREMLFSALRDIVYTESELASQ
RIDLESSQGITDYVFHLLRNARTLRPGVEPKMVVCWGGHSISTEEYQYTKKVGHELGLRK
LDVCTGCGPGVMKGPMKGATIAHAKQRMHGSRYLGLTEPGIIAAEAPNPIVNELVILPDI
EKRLEAFVRVGHGIIIFPGGAGTAEEFLYLLGILMHPDNHDLPFPVVLTGPRSAEPYLQQ
LHAFVGATLGEAAHRLYEIIIDDPAEVARHMVEGLKAVKQFRRERNDAFHFNWLLKIEES
FQRPFDPTHQAMADLDLRRELPPHELAANLRRAFSGIVAGNVKDKGIRLIEEHGPYQIHG
DSAVLDPLGRLLQAFVDQHRMKLPGGAAYVPCYQVAT