Protein Info for PP_3631 in Pseudomonas putida KT2440

Annotation: putative signal transduction protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 216 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 184 to 205 (22 residues), see Phobius details TIGR04211: SH3 domain protein" amino acids 40 to 215 (176 residues), 141.6 bits, see alignment E=1.2e-45 PF08239: SH3_3" amino acids 47 to 96 (50 residues), 41.3 bits, see alignment E=7.1e-15

Best Hits

Swiss-Prot: 37% identical to YGIM_SHIFL: Uncharacterized protein YgiM (ygiM) from Shigella flexneri

KEGG orthology group: K07184, SH3 domain protein (inferred from 100% identity to ppu:PP_3631)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GT6 at UniProt or InterPro

Protein Sequence (216 amino acids)

>PP_3631 putative signal transduction protein (Pseudomonas putida KT2440)
MPDSRPNASALPALRIGLIAALVGLAAPVHAEEPASDARWVSDSLSTYVRSGPTDGHRIV
GTLKSGQKLTLLGSQGNYSQVRGQNGDVVWILSNDLQAVPGQNERLPLLDAQVTELTGQL
KTIDDSWKNRVQGMQETLDSRKQLIDELEARNKALNEQLDQSQSDLRDTQARLGDENKQV
MMRYMVYGGSIAGAGLLAGLILPALTRGRKKNDRWF