Protein Info for PP_3628 in Pseudomonas putida KT2440

Annotation: Choline/carnitine/betaine transporter family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 581 transmembrane" amino acids 34 to 54 (21 residues), see Phobius details amino acids 74 to 93 (20 residues), see Phobius details amino acids 114 to 135 (22 residues), see Phobius details amino acids 168 to 188 (21 residues), see Phobius details amino acids 218 to 240 (23 residues), see Phobius details amino acids 255 to 277 (23 residues), see Phobius details amino acids 289 to 312 (24 residues), see Phobius details amino acids 344 to 364 (21 residues), see Phobius details amino acids 375 to 399 (25 residues), see Phobius details amino acids 431 to 459 (29 residues), see Phobius details amino acids 472 to 490 (19 residues), see Phobius details amino acids 502 to 525 (24 residues), see Phobius details PF02028: BCCT" amino acids 37 to 531 (495 residues), 597.9 bits, see alignment E=6.5e-184 TIGR00842: transporter, betaine/carnitine/choline transporter (BCCT) family" amino acids 75 to 531 (457 residues), 536.6 bits, see alignment E=2.3e-165

Best Hits

Swiss-Prot: 50% identical to BETP_CORGL: Glycine betaine transporter BetP (betP) from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3628)

Predicted SEED Role

"Glycine betaine transporter OpuD" in subsystem Choline and Betaine Uptake and Betaine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GT9 at UniProt or InterPro

Protein Sequence (581 amino acids)

>PP_3628 Choline/carnitine/betaine transporter family protein (Pseudomonas putida KT2440)
MAKEHIPETLDLGVTDPSKTAEARQDRHFEGKLDWIVFGFTSLLAIAFVLWGFLNQASLA
SSASSAQSWVILNFGWFFVLTSSLFVVFVLWLAASRYGRVPLGRDGEQPEFRTVSWVAMM
FSAGMGIGLMFFGVAEPLSHYISPPPGSASGQTSEAMQVAMATTLFHWTLHPWAMYAIVG
LVIAYGTFRRGRSQLISAAFRPLIGKHANGPLGRLIDMMAIFATLFGSAASLGLGALQIA
GGLEYNGWIESPGKLFYISIITLLTIAFVTSAVSGIGKGIQWLSNTNMVLALVLAVFVFM
VGPTLLMLNLLPTSLGIYIKMLPEMMARTNASGGEQMNSWLAGWTVFYWAWWISWTPFVG
MFIARISRGRTIRQFVTGVLLVPSLVSLVWFTIFGGAAIEAVREGAFQLANGAVNSNHAL
YQLLDSYPLASATSVLVMILVGIFFVSGADAASLVMGTLSEHGTTAPSRRTVIFWGTLTG
TVAAIMLAIGDPGNPGEALTGLQNLTIVVALPFVVVMVLLCLALYRDLRKDPMMLRHLRG
NELIEKAVLYGAVKHGEEFYIVVQEKKASAKTAAEAEVTRN