Protein Info for PP_3585 in Pseudomonas putida KT2440

Annotation: multidrug efflux transport system - putative membrane fusion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 435 transmembrane" amino acids 12 to 30 (19 residues), see Phobius details TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 69 to 395 (327 residues), 249.9 bits, see alignment E=1.6e-78 PF16576: HlyD_D23" amino acids 90 to 314 (225 residues), 44.5 bits, see alignment E=1.7e-15 PF13533: Biotin_lipoyl_2" amino acids 92 to 139 (48 residues), 56 bits, see alignment 3.9e-19 PF13437: HlyD_3" amino acids 202 to 304 (103 residues), 23.9 bits, see alignment E=9.1e-09

Best Hits

Swiss-Prot: 48% identical to MDTA_KLEP7: Multidrug resistance protein MdtA (mdtA) from Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578)

KEGG orthology group: K07799, putative multidrug efflux transporter MdtA (inferred from 100% identity to ppu:PP_3585)

Predicted SEED Role

"Probable Co/Zn/Cd efflux system membrane fusion protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GY1 at UniProt or InterPro

Protein Sequence (435 amino acids)

>PP_3585 multidrug efflux transport system - putative membrane fusion protein (Pseudomonas putida KT2440)
MQASNSRSLRRWLVGLLILLLVAALAWWLWPAATPAHKEAGGVRGGKGMGMIGGRPGFGG
SSDPVPVRVVPVRIGDFPLYYKALGTVTATNTVNVRSRVAGELVKIHFKEGQRVKAGDLL
AEIDPRPYRIALQQAEGTLAQNQAQLKNAQVDLARYKGLYAEDSIAKQTLDTAEAQVAQF
QGLVKTNQAQVNDARLNLDFTQIRAPISGRVGLRQLDLGNLVAANDTTALVVITQTEPIN
VAFTLPETELSTVLERYRSGASLPVEAWDRGDSKLQSTGVLGSIDNQIDTTTGTLKFKGR
FENKDLALFPNQFVNVRLLADTLKQVVMAPAAAIQFGNDGTFAYVVNEQSTVNVRKLKVG
ASDGENSVILDGLKAGDRLVLEGTDRLREGTKVEVVEDSSQVPTSPGQHLQGQEAKGAAQ
TGEAQPGNAAGKAGA