Protein Info for PP_3581 in Pseudomonas putida KT2440

Annotation: conserved membrane protein of unknown function

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 695 transmembrane" amino acids 6 to 28 (23 residues), see Phobius details amino acids 41 to 66 (26 residues), see Phobius details amino acids 76 to 97 (22 residues), see Phobius details amino acids 107 to 129 (23 residues), see Phobius details amino acids 141 to 161 (21 residues), see Phobius details amino acids 173 to 195 (23 residues), see Phobius details amino acids 216 to 237 (22 residues), see Phobius details PF03707: MHYT" amino acids 52 to 107 (56 residues), 77.3 bits, see alignment 1.1e-25 amino acids 115 to 167 (53 residues), 65.6 bits, see alignment 4.9e-22 amino acids 182 to 245 (64 residues), 30.6 bits, see alignment E=3.9e-11 TIGR00254: diguanylate cyclase (GGDEF) domain" amino acids 260 to 421 (162 residues), 140 bits, see alignment E=3e-45 PF00990: GGDEF" amino acids 263 to 418 (156 residues), 154.4 bits, see alignment E=3.6e-49 PF00563: EAL" amino acids 439 to 674 (236 residues), 253.4 bits, see alignment E=2.9e-79

Best Hits

Swiss-Prot: 66% identical to Y1727_PSEAE: Uncharacterized signaling protein PA1727 (PA1727) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3581)

Predicted SEED Role

"Sensory box/GGDEF family protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GY4 at UniProt or InterPro

Protein Sequence (695 amino acids)

>PP_3581 conserved membrane protein of unknown function (Pseudomonas putida KT2440)
MLTGSYSSSLVLISLCVAILASYTALDLTGRIATAKGRAACLWMGGGALAMGIGVWSMHF
IGMLAFSLPIDLGYDLALTAFSLLIAVLSSGFALWLVSQPSLPALQLGFGALIMGAGIAC
MHYTGMAALRMLPGIDYDPTLFGASLLIAVGASAAALWIAFRLRRHTPYVRQIRGLAAVV
MGVAIVGMHYTGMAAANFPEGSFCGALGGGLQGDSLVYLVLITTLAVLAVALLTSVLDAR
LEARTAELARSLTLANQELTQLALHDTLTDLPNRTLLADRIEQAIAKVAEQGGCFALMFI
DLDGFKPVNDAFGHHIGDLLLKAVAARLRGHLHSQDTLARIGGDEFVLLVELQEPNDAMD
VAVKQVNLVSRPFRVAEHDLQLSASLGIVLYPGNGQDQHELLRNADAAMYHAKSAGKNGY
SFFDVSMNSNARQQLQLLQDLRLALEQRQFRLHYQPKFDAQACQPIGAEALLRWEHPQQG
LLLPDRFIGLAEKTGLIIPIGEWVLTEACRQMRQWLDQGHHGWRMAVNLSAIQFCHAGLV
DSVARALQQNGLPANCLTLEITETTAMHDADASLTVLQRLSDMGVDLSIDDFGTGYSSLM
YLKRLPANELKIDRGFVRDLEQDSDDAAIVSAIVALGQALGLRIVAEGVETDKQQDFLTR
LGCDSLQGYLLGQPVPAEQFMSKLQAMGPELTAAG