Protein Info for PP_3569 in Pseudomonas putida KT2440

Annotation: quinate dehydrogenase (quinone)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 805 transmembrane" amino acids 12 to 31 (20 residues), see Phobius details amino acids 42 to 60 (19 residues), see Phobius details amino acids 67 to 90 (24 residues), see Phobius details amino acids 96 to 113 (18 residues), see Phobius details amino acids 125 to 145 (21 residues), see Phobius details TIGR03074: membrane-bound PQQ-dependent dehydrogenase, glucose/quinate/shikimate family" amino acids 18 to 805 (788 residues), 1114.5 bits, see alignment E=0 PF01011: PQQ" amino acids 228 to 253 (26 residues), 27.5 bits, see alignment (E = 1.9e-10) amino acids 450 to 480 (31 residues), 26.4 bits, see alignment (E = 4.2e-10) amino acids 500 to 533 (34 residues), 23.2 bits, see alignment (E = 4.5e-09) amino acids 734 to 769 (36 residues), 21.3 bits, see alignment (E = 1.7e-08) PF13360: PQQ_2" amino acids 682 to 769 (88 residues), 20.8 bits, see alignment E=2.7e-08

Best Hits

Swiss-Prot: 65% identical to QUIA_ACIAD: Quinate/shikimate dehydrogenase (quinone) (quiA) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K05358, quinate dehydrogenase (quinone) [EC: 1.1.5.8] (inferred from 100% identity to ppu:PP_3569)

MetaCyc: 65% identical to quinate/shikimate dehydrogenase (quinone) (Acinetobacter baylyi ADP1)
1.1.99.25-RXN [EC: 1.1.5.8]; 1.1.5.8 [EC: 1.1.5.8]

Predicted SEED Role

"Quinate/shikimate dehydrogenase [Pyrroloquinoline-quinone] (EC 1.1.99.25)" in subsystem Chorismate Synthesis or Quinate degradation (EC 1.1.99.25)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.8 or 1.1.99.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GZ6 at UniProt or InterPro

Protein Sequence (805 amino acids)

>PP_3569 quinate dehydrogenase (quinone) (Pseudomonas putida KT2440)
MKETPRASGATNFILVGLGVIIALLGLLLAAGGVKLAGLGGSWYFLIGGLAMAIAGVLIA
RRKKAGAWLYAVFLVGTAIWALIDAGLVFWPLFSRLFMFGAIGMVVALVYPLLARANGAS
AGRGAYGVAGVMAVVLVVAVGNMFVAHPSVAPTGKGPGMTPVETGKEQKDWAHYGNTEGG
SRFAALDQINRDNVNKLKVAWTYQTGDVAISDGNGAEDQLTPLQIGSKVFICTPHNNLIA
LDADTGKELWKNEINAQSKVWQRCRGMAYFDATAAIAQPTQPNSSPITGVSVAAGANCQR
RLLTNTIDGRLIAVDADTGEFCQGFGNNGQVDLKAGLGDVPDSYYQLSSAPLMAGTTVVV
GGRVADNVQTDMPGGVIRGFDVITGEMRWAFDPGNPEDRQAPQGDKTYVRSTPNSWAPMS
YDPAMNTVFLPMGSSSTDIYGVERSKLDHTYGASVLALDATTGNQKWVFQTVHNDLWDFD
LPMQPSLIDFTKDDGQSVPAVVIGTKAGQIYVLDRATGKPLTQVDEVPVKPSNIPNEPYS
PTQPKSVGMPQIGAQTLTESDMWGATPYDQLLCRIDFKKMRYDGLYTAPGTDLSLSFPGS
LGGMNWGSISTDPVHGFIFVNDMRLGLWIQMIPSQNKGGAASGGEALNTGMGAVPLKGTP
YAVNKNRFLSVAGIPCQAPPFGTLTAIDMKTRQVAWQVPVGTVEDTGPLGIRMHLPIKIG
LPTLGGTLSTQGGLVFIAGTQDFYLRAYDSSNGNEIWKARLPVGSQGGPMTYVSPKTGKQ
YVVITAGGARQSTDRGDYVISYALP