Protein Info for PP_3566 in Pseudomonas putida KT2440

Annotation: Major facilitator family transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 399 transmembrane" amino acids 18 to 37 (20 residues), see Phobius details amino acids 56 to 76 (21 residues), see Phobius details amino acids 85 to 104 (20 residues), see Phobius details amino acids 111 to 132 (22 residues), see Phobius details amino acids 144 to 163 (20 residues), see Phobius details amino acids 173 to 194 (22 residues), see Phobius details amino acids 227 to 247 (21 residues), see Phobius details amino acids 256 to 275 (20 residues), see Phobius details amino acids 286 to 304 (19 residues), see Phobius details amino acids 310 to 329 (20 residues), see Phobius details amino acids 348 to 369 (22 residues), see Phobius details amino acids 375 to 394 (20 residues), see Phobius details PF07690: MFS_1" amino acids 24 to 322 (299 residues), 119 bits, see alignment E=3.4e-38 amino acids 228 to 395 (168 residues), 52.6 bits, see alignment E=5.5e-18 PF06779: MFS_4" amino acids 41 to 391 (351 residues), 30.1 bits, see alignment E=4.9e-11 PF00083: Sugar_tr" amino acids 51 to 194 (144 residues), 31.6 bits, see alignment E=1.3e-11

Best Hits

Swiss-Prot: 46% identical to YDER_BACSU: Uncharacterized MFS-type transporter YdeR (ydeR) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3566)

Predicted SEED Role

"L-Proline/Glycine betaine transporter ProP" in subsystem Proline, 4-hydroxyproline uptake and utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88GZ9 at UniProt or InterPro

Protein Sequence (399 amino acids)

>PP_3566 Major facilitator family transporter (Pseudomonas putida KT2440)
MNPYLATAQPAAASLSRALVVLLAFCCGAIVANIYYAQPIVGLIAPDLGLSTEHASLIVS
LTQLGYALGLLLLVPLADLLENRRLMVATAVLACVSLLLAGTSSSGQGQLFLGYALLIGF
SSVSVQMLIPLAAHLAPEQQRGRVVGNIMGGLLLGILLARPLSSVVADHFGWRAVFIGAA
GVMLAIILLLSLTLPQRRPEHKASYTGLMLSLLTLLRRYPLLRQRSLYQALMFAAFSLYW
TAVPMALAGEHGLSQSQIAVFALVGAVGAVAAPLAGRLADAGHARAASLLALLLAPAALL
LGLTVPGYSVIGLGLTGVLLDFAVQMNMVIGQREVYALDPASRGRLNAVYMTSIFLGGAL
GSAVASALFSQFGWQGVALVGAGLPGVALVVFLLKARRG