Protein Info for PP_3546 in Pseudomonas putida KT2440

Annotation: Sensory box histidine kinase/response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 688 PF08448: PAS_4" amino acids 64 to 153 (90 residues), 28.5 bits, see alignment E=3.1e-10 amino acids 183 to 294 (112 residues), 72.1 bits, see alignment E=8.8e-24 PF00512: HisKA" amino acids 317 to 381 (65 residues), 28.7 bits, see alignment E=2.2e-10 PF02518: HATPase_c" amino acids 425 to 544 (120 residues), 63.4 bits, see alignment E=5.2e-21 PF00072: Response_reg" amino acids 571 to 683 (113 residues), 67.4 bits, see alignment E=2.5e-22

Best Hits

KEGG orthology group: None (inferred from 100% identity to ppu:PP_3546)

Predicted SEED Role

"Sensory box sensor histidine kinase/response regulator (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.3.-

Use Curated BLAST to search for 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88H19 at UniProt or InterPro

Protein Sequence (688 amino acids)

>PP_3546 Sensory box histidine kinase/response regulator (Pseudomonas putida KT2440)
MPSRDLNSLRQQVEDLQRQNTLLEQRLASLQAHDQDFYRSLFDTMDEGFCIIEFFDGPHG
PLSDYVHVLANAAYAKHAGIPNVVGQKLREMVPDEADDWVARYGAVLRTGEPLHFEQELI
ATGHVLSVTTFRIEPAEKRQVAVLFKDVTERRRAEQALQRLNEELEQRVNAALAERRLFA
ELVDHSVVNVHVVDKDLRWLAVNRQARHDFHLLYGRTPEIGDYLPGLFEDGTAGQTPILP
MWQRALAGEQFIEIGTFGKPPAVRHYELRFNALRDPKGEVQGAFLFAYDISERVQEQERL
AKAEAALRQAQKMEAVGQLSGGIAHDFNNLLGGILGAQELMRQRLDQSRFDALAPLLELS
SSAAQRASSLVHRLLAFSRQQTLQPCSTQVATLVAGMEDLLRRTIGPAITLSSRFASQLW
PTFIDPPQLESALLNLCINARDAMPAGGVIEIIGDNLLLDDEQAVALELPASEFVRLSIV
DNGSGMSAEVVERAVEPFFTTKPMGQGTGLGLSMTYGFVRQSGGQLRVLSVPGEGTRIEL
LLPRHHEQAQAPISKPPRTLLQRSSEAAKRILLVEDQTALRLVIGEVLEELGYRVDAFEN
GPSALTHLQSGERPDLLLSDVGLPGGLNGRQVAERFRERYPDLKVLLITGYDESAAFSDG
QPLQGTLVLTKPFELEALAERVRELLEP