Protein Info for PP_3495 in Pseudomonas putida KT2440

Annotation: tRNA (adenine(22)-N(1))-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 230 PF12847: Methyltransf_18" amino acids 6 to 157 (152 residues), 171.2 bits, see alignment E=1.6e-54 PF04816: TrmK" amino acids 23 to 206 (184 residues), 207.1 bits, see alignment E=2.2e-65

Best Hits

KEGG orthology group: K06967, (no description) (inferred from 100% identity to ppu:PP_3495)

Predicted SEED Role

"Putative tRNA-m1A22 methylase"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88H66 at UniProt or InterPro

Protein Sequence (230 amino acids)

>PP_3495 tRNA (adenine(22)-N(1))-methyltransferase (Pseudomonas putida KT2440)
MNEQTLSRRLERVAAHVPQGARLADIGSDHGYLPVALMLRGVLEAAVAGEVAQTPFASAQ
RNVRRNGLQEQVTVRLADGLTAVEPQDRISVVSICGMGGDTMCEILEAGKQRLGGVTRLV
LQPNGGERELRQWLAGNGYQIVSEELLRENRFDYEIIVAEPGRGVYSAEQLYFGPVLMQE
KSEAFVVKWRRMLRQKQQTLANFQRARDAVPQAKIDDFNQQVGWITQVLA