Protein Info for PP_3478 in Pseudomonas putida KT2440

Annotation: putative Secretion protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 621 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF07660: STN" amino acids 210 to 260 (51 residues), 31.1 bits, see alignment 5e-11 PF03958: Secretin_N" amino acids 274 to 330 (57 residues), 36.3 bits, see alignment 1.6e-12 PF00263: Secretin" amino acids 396 to 561 (166 residues), 137.7 bits, see alignment E=9.4e-44

Best Hits

KEGG orthology group: K02453, general secretion pathway protein D (inferred from 100% identity to ppu:PP_3478)

Predicted SEED Role

"Predicted secretion system X protein GspD-like"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88H83 at UniProt or InterPro

Protein Sequence (621 amino acids)

>PP_3478 putative Secretion protein (Pseudomonas putida KT2440)
MKSSKLCKPAPFLLVALCVAIAGCGSSAVRQDSDQLMKEGQYEAGIARLEEALRDDPRDT
ELNIALAHSRQAAVEALIGQADSDRVRHDFANARMGYGRVLTLEPNNRRAQEGIRQLELI
RTLDERIALGQAALRQGDLFGAERYMREVLRLDPQNQKGMTLRSDIENVQARTAQPYPQL
RSKLERPVTLEFRDANLKTIFEVLSQVAGINFIFDKDLRPDMKATIFVREVRIEDAIALL
LEQNQLRQKIVNDNTVMVYPDSPQKTKDYQELVMRTFYLTSIDANTALNMVKTMLKTRDV
FVDERLNTLTMRDTSDAVRMAEKLLQSQDQSNPEVVLEVEVMEVATSRILDLGLQWPNTF
GVLSDDGNPVSVLDQLKGINSSRISIAPAPQAKINAQDKDINTLASPVIRVSNREQARIH
IGQRVPIISATSVPSTQGPVITESVTYLDVGLKLEVQPTVHLNNEVAIKVALEVSNATPL
EATRQGTIPVQVDTRNAQTSLRLHDGETQVLAGLVRNDHNASGNKIPGLGDIPGLGRLFG
SNKDDMSKSELVLAITPRIVRNLPYQSPSDMEFSTGTESAMQVRQMAPLPPMDVPGNAPT
TEEPVVDSQMAVAPVNGSPRP