Protein Info for PP_3453 in Pseudomonas putida KT2440

Annotation: putative Sensor protein RstB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 transmembrane" amino acids 7 to 31 (25 residues), see Phobius details amino acids 137 to 159 (23 residues), see Phobius details PF00512: HisKA" amino acids 212 to 273 (62 residues), 31.7 bits, see alignment E=1.3e-11 PF02518: HATPase_c" amino acids 323 to 431 (109 residues), 84 bits, see alignment E=1.1e-27

Best Hits

KEGG orthology group: K02484, two-component system, OmpR family, sensor kinase [EC: 2.7.13.3] (inferred from 100% identity to ppu:PP_3453)

Predicted SEED Role

"Sensory histidine kinase QseC" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HA7 at UniProt or InterPro

Protein Sequence (433 amino acids)

>PP_3453 putative Sensor protein RstB (Pseudomonas putida KT2440)
MLKILVRLYLVIIVAYAGALLFIPDTIVGLFRDRFMAYNLDQAKGVQALIVRQFRQAPRE
QWPAVEQELASDFAPLELQLLRRDQAGLSDEEQARLEQGLYAVRIAEWGYYETVLAPLDK
EWLIRLHSPPDPLDINVLSWGVTVLIGAAMLGCLLLWVWPHWRDLERLKETARRLGQGQM
AERTHISPHSNIGELAGVFDTMASDLERHVNQQRELLNAVSHELRTPLTRLDFGLVLLFD
EVPPASRKRLLELVGHVRELDELVLELLSYSRLYNADQARERVEVSLLELVDSVLGGFAE
ELDGRGIQWEVRAEGELPRFVLDPRLTARAVQNLVRNAMRYCDESLLLRLRLEADGACLL
TVEDDGIGVPVEERERVFQPFYRLDRSRDRNTGGFGLGLAISRRAIEGQGGTLTLAQSAL
GGAQFRIRLPAGG