Protein Info for PP_3448 in Pseudomonas putida KT2440

Annotation: bacteriophage N4 receptor, inner membrane subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 724 transmembrane" amino acids 15 to 41 (27 residues), see Phobius details amino acids 363 to 388 (26 residues), see Phobius details amino acids 399 to 420 (22 residues), see Phobius details PF13641: Glyco_tranf_2_3" amino acids 71 to 334 (264 residues), 115.2 bits, see alignment E=7.4e-37 PF13632: Glyco_trans_2_3" amino acids 166 to 384 (219 residues), 140.2 bits, see alignment E=1.2e-44 PF05157: MshEN" amino acids 540 to 624 (85 residues), 47 bits, see alignment E=3.2e-16

Best Hits

KEGG orthology group: K11740, bacteriophage N4 adsorption protein B (inferred from 100% identity to ppu:PP_3448)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HB2 at UniProt or InterPro

Protein Sequence (724 amino acids)

>PP_3448 bacteriophage N4 receptor, inner membrane subunit (Pseudomonas putida KT2440)
MSLVFIDFLTYLLFGLKLLAITLASLMFLLGLDDLFIDLCYWGRTLIRRFRIYGKFEKAD
EKRLYEVPEKPLAIMVPAWNEVGVVGEMARLAASTIDYENYQIFVGTYPNDPQTQADVDA
VCLHFPNVHKVVCARPGPTSKADCLNNIIDALLRFQQDAGIEFAGFILHDAEDVISPMEL
RLFNYLLPSKDMIQIPVYPYAPEWKGFTAGHYVDEFAENHGKDVIVREALTGQVPSAGVG
TCFSRRAISALLEDGDGIAFDVQSLTEDYDIGFRLKQKGMKCIFARYSITDPALTLKQDW
RPGMSREFSQVICVREHFPRDWQHAIRQKSRWIVGIVFQGTSNLGWSRKGALNYFLWRDR
RGLFAYLLSFLVNLLLLVLLAMWLVTVIAPESWRFMSILSDSWLLSTLLWLNGLMLLNRL
FQRAWFVTRFYGIGEGLLSAPRMMWSNFVNFFANLRALRQVMEMGDSRRVAWDKTTHEFP
AIATPARTPLGQRLVAKGLITDEQLQQAITSPVRRRLGRELLLRGWLNSEQLVATLAEQL
DLPWAPLNPFKLSPQLIAKLPRKLATHYGVLPVDEDGGTLILASESPVSQVSLGVISRQL
KRPVKARLAPQGRVTLGLRYHYPSPWQHQETRDMLAVLERHQDDEALLERVSHHQVMLGA
LLQVRGMVPVTLFNQALIDFDPEHQSLGEHLIARGIITRQVLEQALIEQASEQQVAIDLT
REVA