Protein Info for PP_3436 in Pseudomonas putida KT2440

Annotation: chloramphenicol-sensitive protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 transmembrane" amino acids 5 to 22 (18 residues), see Phobius details amino acids 34 to 51 (18 residues), see Phobius details amino acids 71 to 91 (21 residues), see Phobius details amino acids 102 to 120 (19 residues), see Phobius details amino acids 127 to 143 (17 residues), see Phobius details amino acids 149 to 166 (18 residues), see Phobius details amino acids 178 to 195 (18 residues), see Phobius details amino acids 209 to 229 (21 residues), see Phobius details amino acids 237 to 258 (22 residues), see Phobius details amino acids 268 to 288 (21 residues), see Phobius details TIGR00688: protein RarD" amino acids 3 to 254 (252 residues), 198.7 bits, see alignment E=5.9e-63 PF00892: EamA" amino acids 3 to 139 (137 residues), 28.6 bits, see alignment E=7.6e-11

Best Hits

Swiss-Prot: 44% identical to Y1281_PASMU: Uncharacterized transporter PM1281 (PM1281) from Pasteurella multocida (strain Pm70)

KEGG orthology group: K05786, chloramphenicol-sensitive protein RarD (inferred from 100% identity to ppf:Pput_2327)

Predicted SEED Role

"Protein rarD"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HC4 at UniProt or InterPro

Protein Sequence (295 amino acids)

>PP_3436 chloramphenicol-sensitive protein (Pseudomonas putida KT2440)
MSKGIVSSVMASCLFAVMYFYTSLLKPLDGEEIFGWRTLLTLPCLTLFMLVSKDWKRVGE
LMGRVKRTPVLLLGMVGTSWLMGVQLWLFLWAPLHGRSLEVSMGYFLLPLAMVLTGRLVY
GERLSRLQTVAVACAALGVGHELYQNGSFAWETLLVTIGYPVYFVLRRRCRTDNLGGLWC
DMCLLLPWALYFVIQSPLSSADLQAHPGLYVLVPILGAISASALIAYVLASRMLPFSLFG
LLSYVEPVLLVGVALLLGETIGPDQWLTYLPIWAAVLVLVLEGFKHLLRQRRRSV