Protein Info for PP_3426 in Pseudomonas putida KT2440

Annotation: Multidrug efflux RND transporter MexF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1059 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details transmembrane" amino acids 342 to 362 (21 residues), see Phobius details amino acids 369 to 389 (21 residues), see Phobius details amino acids 396 to 419 (24 residues), see Phobius details amino acids 440 to 461 (22 residues), see Phobius details amino acids 472 to 499 (28 residues), see Phobius details amino acids 548 to 565 (18 residues), see Phobius details amino acids 880 to 899 (20 residues), see Phobius details amino acids 906 to 928 (23 residues), see Phobius details amino acids 934 to 957 (24 residues), see Phobius details amino acids 982 to 1001 (20 residues), see Phobius details amino acids 1011 to 1037 (27 residues), see Phobius details PF00873: ACR_tran" amino acids 3 to 1038 (1036 residues), 1270.8 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 3 to 1052 (1050 residues), 1182.7 bits, see alignment E=0 PF02355: SecD_SecF" amino acids 342 to 499 (158 residues), 23.5 bits, see alignment E=5.2e-09 PF03176: MMPL" amino acids 348 to 499 (152 residues), 38.9 bits, see alignment E=7.9e-14 amino acids 880 to 1048 (169 residues), 24.3 bits, see alignment E=2.3e-09

Best Hits

KEGG orthology group: None (inferred from 86% identity to avn:Avin_33870)

Predicted SEED Role

"Multidrug efflux transporter MexF"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HD4 at UniProt or InterPro

Protein Sequence (1059 amino acids)

>PP_3426 Multidrug efflux RND transporter MexF (Pseudomonas putida KT2440)
MNFSKFFITRPIFAAVLSLVLLIAGSISLFQLPISEYPEVVPPTVVVRANFPGANPKVIG
ETVAAPLEQAITGVENMLYMSSQSTADGKLTLTITFALGTDLDNAQVQVQNRVTRTQPKL
PEEVTRIGITVDKASPDLTMVVHLTSPDNRYDMLYLSNYAILNIKDELARLGGVGDVQLF
GMGDYSLRVWLDPNKTASRNLTASDVVAAIREQNRQVAAGQLGAPPAPGSTSFQLSINTQ
GRLVNEEEFENIIIRAGADGEITRLKDIARVELGSSQYALRSLLNNQPAVAIPIFQRPGS
NAIEISDEVRAKMAELKKDFPEGMDYSIVYDPTIFVRGSIEAVVHTLFEALVLVVLVVIL
FLQTWRASIIPLLAVPVSLIGTFAVMHLFGFSLNALSLFGLVLAIGIVVDDAIVVVENVE
RNIGLGLKPLEATQKAMSEVTGPIIATALVLCAVFVPAAFISGLTGQFYKQFALTIAIST
VISAFNSLTLSPALAAVLLKDHHAPKDRFSRFLDKLLGSWLFSPFNRFFDRASHSYVGGV
RRVIRSSGIALFVYAGLMGLTYLGFSSTPTGFVPAQDKQYLVAFAQLPDAASLDRTEAVI
KRMSEIALKQPGVADSVAFPGLSINGFTNSPNSGIVFTPLKPFDERKDPSQSAAAIAAAL
NAQFADIQDAYIAIFPPPPVQGLGTIGGFRLQIEDRGNLGYEALYKETQNIIAKSHNVPE
LAGLFTSYQVNVPQVDAAIDREKAKTHGVAITDIFDTLQVYLGSLYTNDFNRFGRTYQVN
VQAEQQFRLDAEQIGQLKVRNNLGEMIPLATFLKVSDTSGPDRVMHYNGFITAEINGAAA
PGYSSGQAEAAIEKLLKEELPNGMTFEWTDLTYQQILSGNTALLVFPLCVLLAFLVLAAQ
YESWSLPLAVILIVPMTLLSAITGVIVSGGDNNIFTQIGLIVLVGLACKNAILIVEFAKD
EQAKGLDPLAAVLEACRLRLRPILMTSIAFIMGVVPLVFSSGAGSEMRHAMGVAVFSGMI
GVTVFGLFLTPVFFFLIRRFVERRQARKAEHAQVLENHA