Protein Info for PP_3424 in Pseudomonas putida KT2440

Annotation: Type II secretion pathway protein XcpS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 transmembrane" amino acids 166 to 192 (27 residues), see Phobius details amino acids 213 to 241 (29 residues), see Phobius details amino acids 273 to 294 (22 residues), see Phobius details amino acids 375 to 395 (21 residues), see Phobius details PF00482: T2SSF" amino acids 70 to 193 (124 residues), 101.2 bits, see alignment E=2.1e-33 amino acids 273 to 394 (122 residues), 86.6 bits, see alignment E=7.1e-29

Best Hits

KEGG orthology group: K02455, general secretion pathway protein F (inferred from 100% identity to ppu:PP_3424)

Predicted SEED Role

"General secretion pathway protein F / Type II secretory pathway, component PulF / Type IV fimbrial assembly protein PilC" in subsystem Type IV pilus

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q88HD6 at UniProt or InterPro

Protein Sequence (403 amino acids)

>PP_3424 Type II secretion pathway protein XcpS (Pseudomonas putida KT2440)
MAVRRAAMRYSLKALGRQGVVQLQIDAEDADQARRQAEDQGLRVLSLRSSGGALRGMAWR
REAAFDLVLFSQELSTLLNAGLPLIDALESLAEKSPAATARKVLAELVRQLYEGRSLSQA
LGQQPRVFPPLYVALVQSSERTGALGDALTRYISYRQRLDLVRQKLVGASVYPLLLLLVG
GGVVLFLLGYVVPRFSQVFEGMGTELPWLSRVLMQVGLFLHAQQAPLALGTVGGVAALWL
LRRHPRVRYWASCQLRRLPALHQRLMMYELARFYRSLGILLQGGIPILTAMGMARGLLGN
AAAQGLEQASQRVGEGLPLSDALAAGHLVTPVSLRLLRAGEQSGNLGEMLERCADFHDQE
IGRWVEWFVKLFEPLLMTFIGLLIGLIVILMYMPIFELASSIH